The efficiency of spermatogenesis and the support capacity of Sertoli cells in Characiformes

Autor(es)

Rodrigues, Maira da Silva ^[1] ; de Siqueira-Silva, Diogenes Henrique ^[2] ; Quirino, Patricia Postingel ^[1] ; Ninhaus-Silveira, Alexandre ^[3] ; Verissimo-Silveira, Rosicleire ^[3]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Fac Engn Ilha Solteira, Dept Biol & Zootecnia, LINEO Lab Ictiol Neotrop, Ave Brasil, Ctr 56, BR-15385000 Sao Paulo - Brazil

^[2] UNIFESSPA Univ Fed Sul & Sudeste Para, IESB, Fac Ciencias Saude & Biol FACISB, Folha 31, Quadra 07, Lote Especial S-N, BR-68507590 Nova Mamba, PA - Brazil

^[3] UNESP Univ Estadual Paulista, Dept Biol & Zootecnia, LINEO Lab Ictiol Neotrop, Campus Ilha Solteira, Ave Brasil, Ctr 56, BR-15385000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Theriogenology; v. 103, p. 149-152, NOV 2017.
• Citações Web of Science: 2

Resumo

This stereological analysis of the types of germ cells and the number of Sertoli cells per cyst in Astyanax altiparanae testes during spermatogenesis is the first such report in Characiformes. Testes of 25 male A. altiparanae were examined. Based on the number of spermatogonia B per cyst (469.2 +/- 9.92), we estimated that spermatogonia undergo at least nine mitotic divisions before differentiating into primary spermatocytes. There are four spermatogonia types: undifferentiated spermatogonia A{*}, undifferentiated spermatogonia, differentiated spermatogonia, and type B spermatogonia. The number of Sertoli cells increased gradually from 1.41 +/- 0.51 in the single undifferentiated spermatogonium A{*} to 9.25 +/- 0.50 in cysts of spermatocytes in the leptotene/zygotene stage, possibly related to greater complexity of cellular events during the meiotic stage. The number of germ cells rose dramatically from spermatogonia A (1.0 +/- 0) to spermatogonia B (469.2 +/- 9.92); however, the quantity of spermatocytes inside the cysts in the leptotene/zygotene stage decreased (300.6 +/- 6.97) relative to spermatogonia B, representing a loss of approximately 36% of the former number of cells. This was probably the result of apoptosis, which promotes successful development of the remaining cells during sperm production. The support capacity of Sertoli cells increased gradually during spermatogenesis. (C) 2017 Published by Elsevier Inc. (AU)

• Processo FAPESP: 13/24527-8 - Avaliação histológica e estereológica da espermatogênese do Astyanax altiparanae (Characiformes, Characidae)
• Beneficiário: Maira da Silva Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 14/23379-8 - Transplante xenogênico de espermatogônias tronco em Characidae (Teleostei, Characiformes)
• Beneficiário: Rosicleire Veríssimo Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular