Hypothalamic transcriptional expression of the kis-speptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes

Autor(es)

Marcondes, Rodrigo Rodrigues ^[1] ; Carvalho, Katia Candido ^[1] ; Giannocco, Gisele ^[2] ; Duarte, Daniele Coelho ^[1] ; Garcia, Natalia ^[1] ; Soares-Junior, Jose Maria ^[1] ; Cotrim Guerreiro da Silva, Ismael Dale ^[3] ; Maliqueo, Manuel ^[4] ; Baracat, Edmund Chada ^[1] ; Rosa Maciel, Gustavo Arantes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med FMUSP, Disciplina Ginecol, Lab Ginecol Estrutural & Mol LIM 58, Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Med, Lab Endocrinol Mol & Translac, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Escola Paulista Med, Dept Ginecol, Lab Ginecol Mol & Proteom, Sao Paulo, SP - Brazil

^[4] Univ Chile, West Div, Dept Med, Endocrinol & Metab Lab, Santiago - Chile

• Tipo de documento: Artigo Científico
• Fonte: Clinics; v. 72, n. 8, p. 510-514, AUG 2017.
• Citações Web of Science: 3

Resumo

OBJECTIVES: Polycystic ovary syndrome is a heterogeneous endocrine disorder that affects reproductive-age women. The mechanisms underlying the endocrine heterogeneity and neuroendocrinology of polycystic ovary syndrome are still unclear. In this study, we investigated the expression of the kisspeptin system and gonadotropin-releasing hormone pulse regulators in the hypothalamus as well as factors related to luteinizing hormone secretion in the pituitary of polycystic ovary syndrome rat models induced by testosterone or estradiol. METHODS: A single injection of testosterone propionate (1.25 mg) (n= 10) or estradiol benzoate (0.5 mg) (n= 10) was administered to female rats at 2 days of age to induce experimental polycystic ovary syndrome. Controls were injected with a vehicle (n= 10). Animals were euthanized at 90-94 days of age, and the hypothalamus and pituitary gland were used for gene expression analysis. RESULTS: Rats exposed to testosterone exhibited increased transcriptional expression of the androgen receptor and estrogen receptor-b and reduced expression of kisspeptin in the hypothalamus. However, rats exposed to estradiol did not show any significant changes in hormone levels relative to controls but exhibited hypothalamic downregulation of kisspeptin, tachykinin 3 and estrogen receptor-a genes and upregulation of the gene that encodes the kisspeptin receptor. CONCLUSIONS: Testosterone-and estradiol-exposed rats with different endocrine phenotypes showed differential transcriptional expression of members of the kisspeptin system and sex steroid receptors in the hypothalamus. These differences might account for the different endocrine phenotypes found in testosteroneand estradiol-induced polycystic ovary syndrome rats. (AU)

• Processo FAPESP: 13/12830-8 - Avaliação dos efeitos da metformina e do exercício físico sobre o hipotálamo, hipófise e ovário de modelos animais de síndrome dos ovários policísticos
• Beneficiário: Gustavo Arantes Rosa Maciel
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/17417-3 - Expressão de genes relacionados à fisiologia do hormônio liberador de gonadotrofinas (GnRH) em modelo experimental de ratas com síndrome dos ovários policísticos (SOP) induzido por exposição a androgênios
• Beneficiário: Gustavo Arantes Rosa Maciel
• Modalidade de apoio: Auxílio à Pesquisa - Regular