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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hypothalamic transcriptional expression of the kis-speptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes

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Author(s):
Marcondes, Rodrigo Rodrigues [1] ; Carvalho, Katia Candido [1] ; Giannocco, Gisele [2] ; Duarte, Daniele Coelho [1] ; Garcia, Natalia [1] ; Soares-Junior, Jose Maria [1] ; Cotrim Guerreiro da Silva, Ismael Dale [3] ; Maliqueo, Manuel [4] ; Baracat, Edmund Chada [1] ; Rosa Maciel, Gustavo Arantes [1]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Fac Med FMUSP, Disciplina Ginecol, Lab Ginecol Estrutural & Mol LIM 58, Sao Paulo, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Med, Lab Endocrinol Mol & Translac, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Escola Paulista Med, Dept Ginecol, Lab Ginecol Mol & Proteom, Sao Paulo, SP - Brazil
[4] Univ Chile, West Div, Dept Med, Endocrinol & Metab Lab, Santiago - Chile
Total Affiliations: 4
Document type: Journal article
Source: Clinics; v. 72, n. 8, p. 510-514, AUG 2017.
Web of Science Citations: 3
Abstract

OBJECTIVES: Polycystic ovary syndrome is a heterogeneous endocrine disorder that affects reproductive-age women. The mechanisms underlying the endocrine heterogeneity and neuroendocrinology of polycystic ovary syndrome are still unclear. In this study, we investigated the expression of the kisspeptin system and gonadotropin-releasing hormone pulse regulators in the hypothalamus as well as factors related to luteinizing hormone secretion in the pituitary of polycystic ovary syndrome rat models induced by testosterone or estradiol. METHODS: A single injection of testosterone propionate (1.25 mg) (n= 10) or estradiol benzoate (0.5 mg) (n= 10) was administered to female rats at 2 days of age to induce experimental polycystic ovary syndrome. Controls were injected with a vehicle (n= 10). Animals were euthanized at 90-94 days of age, and the hypothalamus and pituitary gland were used for gene expression analysis. RESULTS: Rats exposed to testosterone exhibited increased transcriptional expression of the androgen receptor and estrogen receptor-b and reduced expression of kisspeptin in the hypothalamus. However, rats exposed to estradiol did not show any significant changes in hormone levels relative to controls but exhibited hypothalamic downregulation of kisspeptin, tachykinin 3 and estrogen receptor-a genes and upregulation of the gene that encodes the kisspeptin receptor. CONCLUSIONS: Testosterone-and estradiol-exposed rats with different endocrine phenotypes showed differential transcriptional expression of members of the kisspeptin system and sex steroid receptors in the hypothalamus. These differences might account for the different endocrine phenotypes found in testosteroneand estradiol-induced polycystic ovary syndrome rats. (AU)

FAPESP's process: 13/12830-8 - Effects of metformin and physical activity on the hypothalamus, pituitary and ovary of rat model of polycystic ovary syndrome
Grantee:Gustavo Arantes Rosa Maciel
Support Opportunities: Regular Research Grants
FAPESP's process: 10/17417-3 - Expression of genes related to gonadotropin-releasing hormone (GnRH) physiology in experimental model of rats with polycystic ovary syndrome (PCOS) induced by androgens
Grantee:Gustavo Arantes Rosa Maciel
Support Opportunities: Regular Research Grants