Anti-apoptotic effects of decyl gallate on the induction of apoptosis in A549 pneumocytes by Paracoccidioides brasiliensis gp43

Autor(es):Mostrar menos -

Bernardi, Thais ^[1] ; da Silva, Julhiany de Fatima ^[1] ; Vicentin, Juliana ^[1] ; de Oliveira, Haroldo Cesar ^[1] ; Assato, Patricia Akemi ^[1] ; Marcos, Caroline Maria ^[1] ; Alves de Paula e Silva, Ana Carolina ^[1] ; Moraes da Silva, Rosangela Aparecida ^[1] ; Regasini, Luis Octavio ^[2] ; Siqueira Silva, Dulce Helena ^[3] ; Bolzani, Vanderlan da Silva ^[3] ; Fusco-Almeida, Ana Marisa ^[1] ; Soares Mendes-Giannini, Maria Jose ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Fac Ciencias Farmaceut, Dept Anal Clin, Lab Micol Clin, Campus Araraquara, Araraquara, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Inst Biociencias Letras & Ciencias Exatas, Dept Quim & Ciencias Ambientais, Campus Sao Jose Do Rio Preto, Sao Jose Do Rio Preto, SP - Brazil

^[3] Univ Estadual Paulista, UNESP, Inst Quim, Dept Quim Organ, Campus Araraquara, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Medical Mycology; v. 55, n. 8, p. 890-894, NOV 2017.
• Citações Web of Science: 1

Resumo

Apoptosis is considered an escapemechanism from the host immune system for the fungus Paracoccidioides spp, and it serves as a vehicle for entry into macrophages without stimulating microbicidal activities. Recently, gp43 of P. brasiliensis was demonstrated to be involved in this process. Therefore, as a new therapeutic alternative, it is very important to study compounds that could reduce the modulation of the induction of apoptosis caused by this fungus. Decyl gallate (G14) is a known antifungal compound, and we decided to investigate its anti-apoptotic properties. Our results demonstrate that G14 was effective against apoptosis induced by gp43, as observed in epithelial cells, and led to a reduction in DNA damage, Bak down-regulation and Bcl-2 up-regulation. Together, these data show that G14 presents promising anti-apoptotic activity. (AU)

• Processo FAPESP: 11/18038-9 - Caracterização e análise funcional do gene da proteína 14-3-3 envolvida no processo de adesão do complexo Paracoccidioides sp. e identificação de alvos potenciais na terapia da paracoccidioidomicose
• Beneficiário: Maria José Soares Mendes Giannini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/14023-8 - Uso de peptídeos com atividade anti-adesiva em Paracoccidoides spp. na terapêutica e profilaxia da paracoccidioidomicose
• Beneficiário: Haroldo Cesar de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/17048-4 - Sistema de dois componentes de transdução de sinais (TCST) como novo alvo para o delineamento de peptídeos antifúngicos
• Beneficiário: Caroline Maria Marcos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado