An automated and self-cleaning nano liquid chromatography mass spectrometry platform featuring an open tubular multi-hole crystal fiber solid phase extraction column and an open tubular separation column

Autor(es)

da Silva, Meire Ribeiro ^{[1, 2]} ; Brandtzaeg, Ole Kristian ^[2] ; Vehus, Tore ^{[2, 3]} ; Lancas, Fernando Mauro ^[1] ; Wilson, Steven Ray ^[2] ; Lundanes, Elsa ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Chem Sao Carlos, Sao Carlos, SP - Brazil

^[2] Univ Oslo, Dept Chem, Post Box 1033, NO-0315 Oslo - Norway

^[3] Univ Agder, Dept Engn Sci, Grimstad - Norway

• Tipo de documento: Artigo Científico
• Fonte: Journal of Chromatography A; v. 1518, p. 104-110, OCT 6 2017.
• Citações Web of Science: 7

Resumo

An open tubular (OT) sample preparation/separation platform was developed. A multi-channel polymer layer open tubular (mPLOT) solid phase extraction (SPE) column was prepared by wall-coating the 126 channels (8 mu m inner diameter (ID) each) of a crystal fiber capillary with an organic polymer, namely poly(styrene-co-octadecene-co-divinylbenzene) (PS-OD-DVB). The mPLOT SPE was coupled online with a 10 mu m x 2m poly(styrene-co-divinylbenzene) (PS-DVB) OT liquid chromatography column with nanospray mass spectrometry (OTLC-MS). Compared to using monolithic/particle-packed SPEs, mPLOT-SPE-OTLC allowed both fast loading and sufficient refocusing on the OT analytical column of small model compounds (sulfonamides approximate to 300 Da). Using automatic filtration/filter back-flushing (AFFL) plumbing, the mPLOT SPE column gave a constant and low back-pressure approximate to 35 bar at 0.5 mu L/min. Surprisingly large sample volumes (10 mu L) were possible to be injected using a 12 cm mPLOT. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 15/12306-2 - Preparação e avaliação de colunas tubulares abertas porosas (PLOT) para separação de sulfonamidas e seus metabólitos acetilados em amostras alimentícias por nano-LC-MS/MS
• Beneficiário: Meire Ribeiro da Silva
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado