Biomolecular corona formation: nature and bactericidal impact on surface-modified silica nanoparticles

Autor(es)

Emer, Maiara ^[1] ; Cardoso, Mateus Borba ^[1]

Afiliação do(s) autor(es)

^[1] LNLS, CNPEM, Lab Nacl Nanotecnol LNNano, Caixa Postal 6192, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MATERIALS CHEMISTRY B; v. 5, n. 40, p. 8052-8059, OCT 28 2017.
• Citações Web of Science: 5

Resumo

Biological fluids contain a plethora of different components that can coat nanoparticle's surface and critically affect their interaction with living systems. The recent literature has focused efforts on understanding the overall protein role in this nanoparticle's coating (commonly called a protein corona) without considering other components' influence. Here, we report a protocol to study the relationship between biological fluid components excluding proteins (referred to as a biomolecular corona) and nanoparticles. For this purpose, functionalized silica nanoparticles were duly synthesized to experimentally investigate the extent of biomolecular corona adsorption over different surfaces. This adsorption was directly correlated with the silica surface chemical constitution, while the extent of biomolecular corona coating affected the overall surface charge and the colloidal stability of the functionalized nanoparticles. In addition, the bactericidal properties of biomolecular corona-coated nanoparticles were tested against two medically relevant bacteria (Escherichia coli and Staphylococcus aureus). Biological results indicated that the presence and the extent of the biomolecular corona affect the bactericidal properties of the nanoparticles. (AU)

• Processo FAPESP: 14/01241-4 - Estudo da interação nanopartícula-bactéria através da funcionalização superficial de estruturas manométricas
• Beneficiário: Maiara Emer
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 14/22322-2 - Funcionalização de nanopartículas de sílica: aumentando a interação biológica
• Beneficiário: Mateus Borba Cardoso
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/25406-5 - Organizando a matéria: colóides formados por associação de surfactantes, polímeros e nanopartículas
• Beneficiário: Watson Loh
• Modalidade de apoio: Auxílio à Pesquisa - Temático