| Texto completo | |
| Autor(es): |
de Camargo, Marcela Rodrigues
[1]
;
Gorgulho, Carolina Mendonca
[1]
;
Rodrigues, Cecilia Pessoa
[2]
;
Penitenti, Marcimara
[3]
;
Longo Frederico, Juliana Cristina
[1]
;
Marchesan Rodrigues, Maria Aparecida
[1]
;
Kaneno, Ramon
[2]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Sao Paulo State Univ UNESP, Sch Med Botucatu, Dept Pathol, Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Microbiol & Immunol, Botucatu, SP - Brazil
[3] Hosp Amaral Carvalho, Lab Flow Cytometry Jau, Jau - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS; v. 32, n. 8, p. 302-308, OCT 2017. |
| Citações Web of Science: | 0 |
| Resumo | |
Aim: Considering the central role of dendritic cells (DCs) on the development of an antitumor immune response, in this study we used a murine model to evaluate how DC transfection with drug-treated tumor cell RNA changes their phenotype, and whether transfection enhances the in vivo effectiveness of a DC-based antitumor vaccine. Materials and Methods: MC-38 colorectal tumor cells were pretreated with the minimum effective concentration of 5-fluorouracil (5-FU), then their total RNA was extracted and transfected into DCs. These DCs were inoculated into C57Bl/6 mice bearing subcutaneous MC-38 tumor. Results: DC transfection with drug-treated tumor RNA increases the percentages of CD40(+) (from 37.6% to 61.4%), CD86(+) (from 39.8% to 53.4%), and major histocompatibility complex class II+ (from 51.2% to 75.3%) cells, whereas significantly increases the in vivo generation of interferon-c producer lymphocytes. Conclusion: These results reinforce our view that treatment of tumor cells with 5-FU induces transcriptional changes that can be transferred to DCs by RNA transfection, enhancing their ability to stimulate an antitumor response. (AU) | |
| Processo FAPESP: | 09/18331-8 - Quimioimunomodulação com agentes antineoplásicos e geração de vacinas de células dendríticas. |
| Beneficiário: | Ramon Kaneno |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 10/06013-9 - Efeito protetor in vivo de vacinas de células dendríticas sensibilizadas com mRNA de células MC-38 pré-tratadas com agentes antineoplásicos em doses efetivas mínimas. |
| Beneficiário: | Marcela Rodrigues de Camargo |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 11/05258-0 - Efeito imunomodulador de baixas concentrações de antineoplásicos |
| Beneficiário: | Juliana Cristina Longo Frederico |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |