| Texto completo | |
| Autor(es): |
Castillo Pedraza, Midian C.
[1]
;
Novais, Tatiana F.
[1]
;
Faustoferri, Roberta C.
[2]
;
Quivey, Jr., Robert G.
[2]
;
Terekhov, Anton
[3]
;
Hamaker, Bruce R.
[3]
;
Klein, Marlise I.
[1]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Sao Paulo State Univ Unesp, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara - Brazil
[2] Univ Rochester, Ctr Oral Biol, Rochester, NY - USA
[3] Purdue Univ, Whistler Ctr Carbohydrate Res, Smith Hall, W Lafayette, IN 47907 - USA
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOFOULING; v. 33, n. 9, p. 722-740, 2017. |
| Citações Web of Science: | 11 |
| Resumo | |
Streptococcus mutans-derived exopolysaccharides are virulence determinants in the matrix of biofilms that cause caries. Extracellular DNA (eDNA) and lipoteichoic acid (LTA) are found in cariogenic biofilms, but their functions are unclear. Therefore, strains of S. mutans carrying single deletions that would modulate matrix components were used: eDNA -Delta lytS and Delta lytT; LTA -Delta dltA and Delta dltD; and insoluble exopolysaccharide -Delta gtfB. Single-species (parental strain S. mutans UA159 or individual mutant strains) and mixed-species (UA159 or mutant strain, Actinomyces naeslundii and Streptococcus gordonii) biofilms were evaluated. Distinct amounts of matrix components were detected, depending on the inactivated gene. eDNA was found to be cooperative with exopolysaccharide in early phases, while LTA played a larger role in the later phases of biofilm development. The architecture of mutant strains biofilms was distinct (vs UA159), demonstrating that eDNA and LTA influence exopolysaccharide distribution and microcolony organization. Thus, eDNA and LTA may shape exopolysaccharide structure, affecting strategies for controlling pathogenic biofilms. (AU) | |
| Processo FAPESP: | 09/54035-4 - EMU: facility para estudos avançados de materiais nanoestruturados e biossistemas / FAMA |
| Beneficiário: | Igor Polikarpov |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |
| Processo FAPESP: | 14/05423-0 - Função de DNA extracelular e de ácidos lipoteicóicos na matriz extracelular de biofilmes cariogênicos |
| Beneficiário: | Marlise Inêz Klein Furlan |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 14/21355-4 - Avaliação da função de DNA extracellular e ácido lipoteicóico nas propriedades estruturais e funcionais da matriz extracelular de biofilmes cariogênicos |
| Beneficiário: | Midian Clara Castillo Pedraza |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |