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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Extracellular DNA and lipoteichoic acids interact with exopolysaccharides in the extracellular matrix of Streptococcus mutans biofilms

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Author(s):
Castillo Pedraza, Midian C. [1] ; Novais, Tatiana F. [1] ; Faustoferri, Roberta C. [2] ; Quivey, Jr., Robert G. [2] ; Terekhov, Anton [3] ; Hamaker, Bruce R. [3] ; Klein, Marlise I. [1]
Total Authors: 7
Affiliation:
[1] Sao Paulo State Univ Unesp, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara - Brazil
[2] Univ Rochester, Ctr Oral Biol, Rochester, NY - USA
[3] Purdue Univ, Whistler Ctr Carbohydrate Res, Smith Hall, W Lafayette, IN 47907 - USA
Total Affiliations: 3
Document type: Journal article
Source: BIOFOULING; v. 33, n. 9, p. 722-740, 2017.
Web of Science Citations: 11
Abstract

Streptococcus mutans-derived exopolysaccharides are virulence determinants in the matrix of biofilms that cause caries. Extracellular DNA (eDNA) and lipoteichoic acid (LTA) are found in cariogenic biofilms, but their functions are unclear. Therefore, strains of S. mutans carrying single deletions that would modulate matrix components were used: eDNA -Delta lytS and Delta lytT; LTA -Delta dltA and Delta dltD; and insoluble exopolysaccharide -Delta gtfB. Single-species (parental strain S. mutans UA159 or individual mutant strains) and mixed-species (UA159 or mutant strain, Actinomyces naeslundii and Streptococcus gordonii) biofilms were evaluated. Distinct amounts of matrix components were detected, depending on the inactivated gene. eDNA was found to be cooperative with exopolysaccharide in early phases, while LTA played a larger role in the later phases of biofilm development. The architecture of mutant strains biofilms was distinct (vs UA159), demonstrating that eDNA and LTA influence exopolysaccharide distribution and microcolony organization. Thus, eDNA and LTA may shape exopolysaccharide structure, affecting strategies for controlling pathogenic biofilms. (AU)

FAPESP's process: 09/54035-4 - Facility for advanced studies of biosystems and nanostructured materials
Grantee:Igor Polikarpov
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 14/05423-0 - Role of extracellular DNA and lipoteichoic acid in the matrix of cariogenic biofilm
Grantee:Marlise Inêz Klein Furlan
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 14/21355-4 - Evaluation of the role of extracellular DNA and lipoteichoic acid in the structural and functional properties of the matrix of cariogenic biofilm
Grantee:Midian Clara Castillo Pedraza
Support Opportunities: Scholarships in Brazil - Master