Low levels of exosomal-miRNAs in maternal blood are associated with early pregnancy loss in cloned cattle

Autor(es):Mostrar menos -

De Bem, T. H. C. ^[1] ; da Silveira, J. C. ^[2] ; Sampaio, R. V. ^[2] ; Sangalli, J. R. ^[2] ; Oliveira, M. L. F. ^[2] ; Ferreira, R. M. ^[3] ; Silva, L. A. ^[2] ; Perecin, F. ^[2] ; King, W. A. ^[4] ; Meirelles, F. V. ^[2] ; Ramos, E. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Ribeirao Preto - Brazil

^[2] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Reprod, Sao Paulo - Brazil

^[4] Univ Guelph, Ontario Vet Coll, Dept Biomed Sci, Guelph, ON - Canada

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 7, OCT 30 2017.
• Citações Web of Science: 4

Resumo

Nuclear reprogramming mediated by somatic cell nuclear transfer (SCNT) has many applications in medicine. However, animal clones show increased rates of abortion and reduced neonatal viability. Herein, we used exosomal-miRNA profiles as a non-invasive biomarker to identify pathological pregnancies. MiRNAs play important roles in cellular proliferation and differentiation during early mammalian development. Thus, the aim of this study was to identify exosomal-miRNAs in maternal blood at 21 days of gestation that could be used for diagnosis and prognosis during early clone pregnancies in cattle. Out of 40 bovine-specific miRNAs, 27 (67.5%) were with low abundance in the C-EPL (Clone - Early pregnancy loss) group compared with the C-LTP (Clone - Late pregnancy) and AI-LTP (Artificial Insemination - Late pregnancy) groups, which had similar miRNAs levels. Bioinformatics analysis of the predicted target genes demonstrated signaling pathways and functional annotation clusters associated with critical biological processes including cell proliferation, differentiation, apoptosis, angiogenesis and embryonic development. In conclusion, our results demonstrate decreased exosomal-miRNAs in maternal blood at 21 days of gestation in cloned cattle pregnancies that failed to reach term. Furthermore, the predicted target genes regulated by these 27 miRNAs are strongly associated with pregnancy establishment and in utero embryonic development. (AU)

• Processo FAPESP: 11/51126-9 - Produção de clones bovinos leiteiros em larga escala comercial
• Beneficiário: Tiago Henrique Camara de Bem
• Modalidade de apoio: Auxílio à Pesquisa - PAPPE / PIPE III
• Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
• Beneficiário: Dimas Tadeu Covas
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 12/50533-2 - GIFT: melhoramento genômico de características relacionadas com a fertilização em gado bovino dinamarquês e brasileiro
• Beneficiário: Marcelo Fábio Gouveia Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/22887-0 - Exossomos e microvesículas contendo miRNAs modulam mudanças epigenéticas durante o cultivo in vitro de gametas e embriões em bovinos
• Beneficiário: Juliano Coelho da Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores