Serological and molecular diagnostic tests for canine visceral leishmaniasis in Brazilian endemic area: one out of five seronegative dogs are infected

Autor(es):Mostrar menos -

Lopes, E. G. ^[1] ; Seva, A. P. ^[1] ; Ferreira, F. ^[1] ; Nunes, C. M. ^[2] ; Keid, L. B. ^[3] ; Hiramoto, R. M. ^[4] ; Ferreira, H. L. ^[3] ; Oliveira, T. M. F. S. ^[3] ; Bigotto, M. F. D. ^[1] ; Galvis-Ovallos, F. ^[5] ; Galati, E. A. B. ^[5] ; Soares, R. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Med Vet Prevent & Saude Anim, Fac Med Vet & Zootecnia, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP - Brazil

^[2] Univ Estadual Paulista, Fac Med Vet, Dept Med Vet, Campus Aracatuba, Aracatuba, SP - Brazil

^[3] Univ Sao Paulo, Dept Med Vet, Fac Zootecnia & Engn Alimentos, Pirassununga, SP - Brazil

^[4] Adolfo Lutz Inst, Ctr Parasitol & Micol, Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EPIDEMIOLOGY AND INFECTION; v. 145, n. 12, p. 2436-2444, SEP 2017.
• Citações Web of Science: 11

Resumo

Euthanasia of infected dogs is one of the measures adopted in Brazil to control visceral leishmaniasis (VL) in endemic areas. To detect infected dogs, animals are screened with the rapid test DPP (R) Visceral Canine Leishmaniasis for detection of antibodies against K26/K39 fusion antigens of amastigotes (DPP). DPP-positives are confirmed with an immunoenzymatic assay probing soluble antigens of promastigotes (ELISA), while DPP-negatives are considered free of infection. Here, 975 dogs from an endemic region were surveyed by using DPP, ELISA and real-time PCR (qPCR) for the diagnosis of VL. When DPP-negative dogs were tested by qPCR applied in blood and lymph node aspirates, 174/887 (19.6%) were positive in at least one sample. In a second sampling using 115 cases, the DPP-negative dogs were tested by qPCR in blood, lymph node and conjunctival swab samples, and 36/79 (45.6%) were positive in at least one sample. Low-to-moderate pairwise agreement was observed between all possible pair of tests. In conclusion, the official diagnosis of VL in dogs in Brazilian endemic areas failed to accuse an expressive number of infected animals and the impact of the low accuracy of serological tests in the success of euthanasia-based measure for VL control need to be assessed. (AU)

• Processo FAPESP: 11/14892-5 - Avaliação da efetividade do emprego de coleira impregnada com inseticida e de vacina recombinante contra leishmaniose visceral canina
• Beneficiário: Estela Gallucci Lopes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/21796-2 - Avaliação da efetividade do emprego de coleira impregnada com inseticida e de vacina de subunidade contra leishmaniose visceral canina
• Beneficiário: Rodrigo Martins Soares
• Modalidade de apoio: Auxílio à Pesquisa - Regular