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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Serological and molecular diagnostic tests for canine visceral leishmaniasis in Brazilian endemic area: one out of five seronegative dogs are infected

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Author(s):
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Lopes, E. G. [1] ; Seva, A. P. [1] ; Ferreira, F. [1] ; Nunes, C. M. [2] ; Keid, L. B. [3] ; Hiramoto, R. M. [4] ; Ferreira, H. L. [3] ; Oliveira, T. M. F. S. [3] ; Bigotto, M. F. D. [1] ; Galvis-Ovallos, F. [5] ; Galati, E. A. B. [5] ; Soares, R. M. [1]
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo, Dept Med Vet Prevent & Saude Anim, Fac Med Vet & Zootecnia, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP - Brazil
[2] Univ Estadual Paulista, Fac Med Vet, Dept Med Vet, Campus Aracatuba, Aracatuba, SP - Brazil
[3] Univ Sao Paulo, Dept Med Vet, Fac Zootecnia & Engn Alimentos, Pirassununga, SP - Brazil
[4] Adolfo Lutz Inst, Ctr Parasitol & Micol, Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, Sao Paulo, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: EPIDEMIOLOGY AND INFECTION; v. 145, n. 12, p. 2436-2444, SEP 2017.
Web of Science Citations: 11
Abstract

Euthanasia of infected dogs is one of the measures adopted in Brazil to control visceral leishmaniasis (VL) in endemic areas. To detect infected dogs, animals are screened with the rapid test DPP (R) Visceral Canine Leishmaniasis for detection of antibodies against K26/K39 fusion antigens of amastigotes (DPP). DPP-positives are confirmed with an immunoenzymatic assay probing soluble antigens of promastigotes (ELISA), while DPP-negatives are considered free of infection. Here, 975 dogs from an endemic region were surveyed by using DPP, ELISA and real-time PCR (qPCR) for the diagnosis of VL. When DPP-negative dogs were tested by qPCR applied in blood and lymph node aspirates, 174/887 (19.6%) were positive in at least one sample. In a second sampling using 115 cases, the DPP-negative dogs were tested by qPCR in blood, lymph node and conjunctival swab samples, and 36/79 (45.6%) were positive in at least one sample. Low-to-moderate pairwise agreement was observed between all possible pair of tests. In conclusion, the official diagnosis of VL in dogs in Brazilian endemic areas failed to accuse an expressive number of infected animals and the impact of the low accuracy of serological tests in the success of euthanasia-based measure for VL control need to be assessed. (AU)

FAPESP's process: 11/14892-5 - Evaluation of the effectiveness of the use of insecticide impregnated collar and recombinant vaccine against canine visceral leishmaniasis
Grantee:Estela Gallucci Lopes
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 11/21796-2 - Evaluation of the effectiveness of the use of insecticide impregnated collar and subunit vaccine against canine visceral leishmaniasis
Grantee:Rodrigo Martins Soares
Support Opportunities: Regular Research Grants