Comparison between conventional indirect ELISA and plasmonic ELISA in detection of IgG anti-Leishmania

• Grant number: 17/25821-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2018
• End date: June 30, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Valéria Marçal Felix de Lima
• Grantee: Matheus Fujimura Soares
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The Visceral Canine Leishmaniasis (VCL) is a zoonosis disease caused by protozoans of Leishmania gender, being Leishmania infantum the most common agent in Brazil. Due to its zoonotic character, many control and prevention methods are applied, standing out the serological methods for detection of sick dogs in the population, and ELISA is the routine test pro diagnosis of VCL. However, part of infected animals is not detected by regular tests, making these animals parasite reservoir in the population. The Plasmonic ELISA (pELISA) arises as an alternative due to its higher sensitivity, and it does not need equipment for reading the results, as result, can be observed with naked eye. Due to its zoonotic importance, the study of new diagnostic methods is important, because it can be a new tool for control of VCL. Therefore, in this work, 180 samples of adult dogs blood, form both sex, collected from Araçatuba region, is going to be used for detection of antibodies anti-Leishmania through conventional ELISA and pELISA, using PCR as the gold standard, comparing, in this way, the sensitivity of each serological method. It's going to be used to 35 blood samples of adult dogs, from both sexes, obtained from laboratory routine of a non-endemic area from the disease (AU)