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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Novel endodontic sealers induce cell cytotoxicity and apoptosis in a dose-dependent behavior and favorable response in mice subcutaneous tissue

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Autor(es):
Silva, L. A. B. [1] ; Azevedo, L. U. [1] ; Consolaro, A. [2] ; Barnett, F. [3] ; Xu, Y. [4] ; Battaglino, R. A. [5] ; Canadas, P. S. [6] ; Holanda de Oliveira, Katharina Morant [1] ; Silva, R. A. B. [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Dent, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Bauru Dent Sch, Dept Oral Pathol, Bauru, SP - Brazil
[3] Albert Einstein Med Ctr, Dept Dent Med, Philadelphia, PA 19141 - USA
[4] Forsyth Inst, Dept Skeletal Biol, Cambridge, MA - USA
[5] Univ Colorado, Sch Med, Dept Phys Med & Rehabil, Denver, CO - USA
[6] Univ Barcelona, Sch Dent, Dept Endodont, Barcelona - Spain
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: CLINICAL ORAL INVESTIGATIONS; v. 21, n. 9, p. 2851-2861, DEC 2017.
Citações Web of Science: 1
Resumo

The objective of the present study is to evaluate the in vitro cytotoxicity and in vivo biocompatibility of two novel endodontic sealers: RealSeal XT1 and Sealapex Xpress on the subcutaneous connective tissue of mice. The cytotoxicity was assessed by cell viability using the MTT assay (one-way ANOVA), trypan blue test (Mann-Whitney) and cell apoptosis by flow cytometer. For the subcutaneous study, polyethylene tubes filled with the sealers were implanted in 70 BALB/c mice: 6 experimental groups (n = 10/group) and 2 control groups with empty tubes (n = 5/group). At the end of experimental periods (7, 21, and 63 days), the tissue was removed and histotechnically processed. Angioblastic proliferation and edema (Fisher's exact test) were evaluated, besides thickness measurement (mu m) of the reactionary granulomatous tissue and neutrophil counts (Kruskal-Wallis and Dunn's post test; Mann-Whitney) (alpha = 0.05). MTT assay, trypan blue, and analysis of apoptotic cells showed a dose-dependent direct effect: the more diluted the sealer, the less cytotoxic. Regarding the angioblastic proliferation and edema, difference between the sealers at 7 and 63 days occurred (p < 0.05). Both endodontic sealers initially promoted perimaterial tissue reaction as a foreign body granuloma and thus stimulated favorable tissue responses. Both sealers showed a dose-dependent effect and promoted satisfactory subcutaneous tissue response; the sealer Sealapex Xpress was less cytotoxic and more biocompatible than RealSeal XT. The step of root canal filling during endodontic treatment is highly important for the preservation of the periapical tissue integrity. Subcutaneous reaction to endodontic sealers enables scientific basis for clinical use. (AU)

Processo FAPESP: 13/21180-7 - Avaliação do comportamento celular e da resposta do tecido conjuntivo subcutâneo de camundongos isogênicos induzidas por cimentos endodônticosndodônticos
Beneficiário:Leopoldo Ulian Azevedo
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica