Full-length genomic and molecular characterization of Canine parvovirus in dogs from North of Brazil

Autor(es):Mostrar menos -

Silva, S. P. ^[1] ; Silva, L. N. P. P. ^[1] ; Rodrigues, E. D. L. ^[2] ; Cardoso, J. F. ^[1] ; Tavares, F. N. ^[3] ; Souza, W. M. ^[4] ; Santos, C. M. P. ^[2] ; Martins, F. M. S. ^[2] ; Jesus, I. S. ^[2] ; Brito, T. C. ^[2] ; Moura, T. P. C. ^[2] ; Nunes, M. R. T. ^[1] ; Casseb, L. M. N. ^[3] ; Silva Filho, E. ^[2] ; Casseb, A. R. ^[2]

Afiliação do(s) autor(es)

^[1] Minist Saude, Ctr Inovacao Tecnol, Inst Evandro Chagas, Ananindeua, PA - Brazil

^[2] Univ Fed Rural Amazonia, Lab Biol Mol, Belem, Para - Brazil

^[3] Inst Evandro Chagas, Dept Virol, Ananindeua, PA - Brazil

^[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Ctr Pesquisa Virol, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetics and Molecular Research; v. 16, n. 3 OCT 3 2017.
• Citações Web of Science: 2

Resumo

With the objective of characterizing Canine parvovirus (CPV) from some suspected fecal samples of dogs collected from the Veterinarian Hospital in Belem city, five positive samples were found by PCR assay and an update molecular characterization was provided of the CPV-2 circulation in Belem. Through sequencing of the complete DNA sequences (NS1, NS2, VP1, and VP2 genes), the CPV-2 strain was identified as CPV-2b (Asn426Asp) circulating in Belem. The CPV-2b strain with a different change at the position Tyr324Leu was detected in all samples assessed and thus reported for the first time for the scientific community. Phylogenetic analysis indicated that Belem CPV-2b and CPV-2a strains would be related to a cluster with samples after the 1990s, suggesting that CPV-2b in Belem originated from CPV2a circulating in Brazil after the 1990s. Potential recombination events were analyzed using RDP4 and SplitsTree4; therefore, results suggest that CPV-2 sequences here described were not potentially recombination events. Continuous monitoring and molecular characterization of CPV2 samples are needed not only to identify possible genetic and antigenic changes that may interfere with the effectiveness of vaccines but also to bring a better understanding of the mechanisms that drive the evolution of CPV-2 in Brazil. (AU)

• Processo FAPESP: 15/05778-5 - Análises de bioinformática de dados de metagenômica viral de animais do Brasil e Antártida
• Beneficiário: William Marciel de Souza
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 12/24150-9 - Pesquisa de vírus em roedores silvestres, mosquitos e carrapatos
• Beneficiário: William Marciel de Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado