Biochemical approaches to assess oxidative stress induced by exposure to natural and synthetic dyes in early life stages in zebrafish

Autor(es)

Abe, Flavia R. ^[1] ; Gravato, Carlos ^{[2, 3]} ; Soares, Amadeu M. V. M. ^{[2, 3]} ; de Oliveira, Danielle P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Aveiro, Dept Biol, Aveiro - Portugal

^[3] Univ Aveiro, CESAM, Aveiro - Portugal

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES; v. 80, n. 23-24, p. 1259-1268, 2017.
• Citações Web of Science: 6

Resumo

Zebrafish early life stages were found to be sensitive to several synthetic dyes widely used in industries. However, as environmental concentrations of such contaminants are often at sublethal levels, more sensitive methods are required to determine early-warning adverse consequences. The aim of this study was to utilize a multibiomarker approach to examine underlying oxidative stress mechanisms triggered by sublethal concentrations of synthetic azo dye Basic Red 51 (BR51), the natural dye erythrostominone (ERY), and its light-degraded product using zebrafish embryos. Biochemical biomarkers included parameters of detoxification and markers of antioxidant system, as well as oxidative damage. Results showed prooxidant mechanisms attributed to BR51 and ERY as evidenced by increased glutathione S-transferase (GST) activity, a phase II detoxification enzyme related to reactive oxygen species detoxification. BR51 also elevated total glutathione (GSH+GSSG) levels and catalase activity. However, both dyes induced oxidative damage as evidenced by elevated lipid peroxidation content. In contrast, when the natural dye was photodegraded, no marked effects were observed for all biomarkers assessed. Data indicate that such dyes are pro-oxidants at sublethal concentrations, predominantly involving GSH and/or related enzymes pathway. (AU)

• Processo FAPESP: 13/01509-4 - Avaliação dos efeitos letais, sub-letais, teratogênicos e genotóxicos de corantes têxteis sobre o desenvolvimento êmbrio-larval de zebrafish (Danio rerio)
• Beneficiário: Danielle Palma de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/27009-0 - Abordagens toxicogenômicas e bioquímicas para avaliação de toxicidade de corante natural e sintético para zebrafish
• Beneficiário: Flavia Renata Abe
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 13/14397-0 - Avaliação ecogenotoxicológica de corante natural extraído de micro-organismo
• Beneficiário: Flavia Renata Abe
• Modalidade de apoio: Bolsas no Brasil - Doutorado