Abe, Flavia R.
Soares, Amadeu M. V. M.
de Oliveira, Danielle P.
Total Authors: 4
 Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
 Univ Aveiro, Dept Biol, Aveiro - Portugal
 Univ Aveiro, CESAM, Aveiro - Portugal
Total Affiliations: 3
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES;
Web of Science Citations:
Zebrafish early life stages were found to be sensitive to several synthetic dyes widely used in industries. However, as environmental concentrations of such contaminants are often at sublethal levels, more sensitive methods are required to determine early-warning adverse consequences. The aim of this study was to utilize a multibiomarker approach to examine underlying oxidative stress mechanisms triggered by sublethal concentrations of synthetic azo dye Basic Red 51 (BR51), the natural dye erythrostominone (ERY), and its light-degraded product using zebrafish embryos. Biochemical biomarkers included parameters of detoxification and markers of antioxidant system, as well as oxidative damage. Results showed prooxidant mechanisms attributed to BR51 and ERY as evidenced by increased glutathione S-transferase (GST) activity, a phase II detoxification enzyme related to reactive oxygen species detoxification. BR51 also elevated total glutathione (GSH+GSSG) levels and catalase activity. However, both dyes induced oxidative damage as evidenced by elevated lipid peroxidation content. In contrast, when the natural dye was photodegraded, no marked effects were observed for all biomarkers assessed. Data indicate that such dyes are pro-oxidants at sublethal concentrations, predominantly involving GSH and/or related enzymes pathway. (AU)