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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biochemical approaches to assess oxidative stress induced by exposure to natural and synthetic dyes in early life stages in zebrafish

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Author(s):
Abe, Flavia R. [1] ; Gravato, Carlos [2, 3] ; Soares, Amadeu M. V. M. [2, 3] ; de Oliveira, Danielle P. [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Aveiro, Dept Biol, Aveiro - Portugal
[3] Univ Aveiro, CESAM, Aveiro - Portugal
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES; v. 80, n. 23-24, p. 1259-1268, 2017.
Web of Science Citations: 6
Abstract

Zebrafish early life stages were found to be sensitive to several synthetic dyes widely used in industries. However, as environmental concentrations of such contaminants are often at sublethal levels, more sensitive methods are required to determine early-warning adverse consequences. The aim of this study was to utilize a multibiomarker approach to examine underlying oxidative stress mechanisms triggered by sublethal concentrations of synthetic azo dye Basic Red 51 (BR51), the natural dye erythrostominone (ERY), and its light-degraded product using zebrafish embryos. Biochemical biomarkers included parameters of detoxification and markers of antioxidant system, as well as oxidative damage. Results showed prooxidant mechanisms attributed to BR51 and ERY as evidenced by increased glutathione S-transferase (GST) activity, a phase II detoxification enzyme related to reactive oxygen species detoxification. BR51 also elevated total glutathione (GSH+GSSG) levels and catalase activity. However, both dyes induced oxidative damage as evidenced by elevated lipid peroxidation content. In contrast, when the natural dye was photodegraded, no marked effects were observed for all biomarkers assessed. Data indicate that such dyes are pro-oxidants at sublethal concentrations, predominantly involving GSH and/or related enzymes pathway. (AU)

FAPESP's process: 13/01509-4 - Assessment of lethal, sublethal, teratogenic and genotoxic effects of textile dyes in zebrafish (Danio rerio) early developmental stages
Grantee:Danielle Palma de Oliveira
Support type: Regular Research Grants
FAPESP's process: 14/27009-0 - Toxicogenomic and biochemical approaches to toxicity assessment of natural and synthetic dyes to zebrafish
Grantee:Flavia Renata Abe
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 13/14397-0 - Ecogenotoxicological evaluation of natural dye extracted from microorganism
Grantee:Flavia Renata Abe
Support type: Scholarships in Brazil - Doctorate