The Role of beta-Adrenergic Overstimulation in the Early Stages of Renal Injury

Autor(es)

de Ponte, Mariana Charleaux ^[1] ; Malavazzi Casare, Fernando Augusto ^[1] ; Costa-Pessoa, Juliana Martins ^[1] ; Cardoso, Vanessa Gerolde ^[1] ; Malnic, Gerhard ^[1] ; Mello-Aires, Margarida ^[1] ; Volpini, Rildo Aparecido ^[2] ; Thieme, Karina ^[3] ; Oliveira-Souza, Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Physiol & Biophys, Lab Renal Physiol, Inst Biomed Sci, Med Sch, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Nephrol Dept, Lab Basic Kidney Dis LIM 12, Med Sch, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Med Sch, Lab Carbohydrates & Radioimmunoassays LIM 18, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Kidney & Blood Pressure Research; v. 42, n. 6, p. 1277-1289, 2017.
• Citações Web of Science: 1

Resumo

Background/Aims: To assess the possible contribution of the beta-adrenergic overstimulation in early stages of renal injury, the present study evaluated, in rats, the effects of the beta-adrenoceptor agonist isoproterenol (ISO) on renal function and morphology, as well as the renal mRNA and protein expression of the NADPH oxidase isoform 4 (Nox 4) and subunit p22(phox), endoplasmic reticulum (ER) stress, pro-inflammatory, pro-apoptotic and renin-angiotensin system (RAS) components. Methods: Wistar rats received ISO (0.3 mg.kg(-1).day(-1) s.c.) or vehicle (control) for eight days. At the end of the treatment, food and water intake, urine output and body weight gain were evaluated and renal function studies were performed. Renal tissue was used for the morphological, quantitative PCR and immunohistochemical studies. Results: ISO did not change metabolic parameters or urine output. However it induced a decrease in renal blood flow and an increase in the filtration fraction. These changes were accompanied by increased cortical mRNA and protein expression for the renal oxidative stress components including Nox 4 and p22(phox); ER stress, pro-inflamatory, pro-apoptotic as well as RAS components. ISO also induced a significant increase in medullar renin protein expression. Conclusion: These findings support relevant information regarding the contribution of specific beta-adrenergic hyperactivity in early stage of renal injury, indicating the reactive oxygen species, ER stress and intrarenal RAS as important factors in this process. (c) 2017 The Author(s) Published by S. Karger AG, Basel (AU)

• Processo FAPESP: 11/14022-0 - Efeitos da interação do sistema-renina-angiotensina-aldosterona e endotelinas-1 e 3 sobre a função renal
• Beneficiário: Fernando Augusto Malavazzi Casare
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/17251-9 - Efeitos da administração crônica de albumina modificada por glicação avançada (AGE) sobre o tecido renal: caracterização do perfil inflamatório, antioxidante e epigenético
• Beneficiário: Karina Thieme
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/19154-0 - Efeito da angiotensina II nos mecanismos celulares envolvidos na injúria de podócitos
• Beneficiário: Vanessa Gerolde Cardoso
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/23087-4 - Estudo molecular e funcional de transportadores de membrana
• Beneficiário: Gerhard Malnic
• Modalidade de apoio: Auxílio à Pesquisa - Temático