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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens

Texto completo
Autor(es):
Caiaffa, Karina Sampaio [1] ; Massunari, Loiane [1] ; Danelon, Marcelle [2] ; Abuna, Gabriel Flores [3] ; Lombardo Bedran, Telma Blanca [4, 5] ; Santos-Filho, Norival Alves [6] ; Palomari Spolidorio, Denise Madalena [4] ; Vizoto, Natalia Leal [7] ; Cilli, Eduardo Maffud [6] ; Duque, Cristiane [2]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Restorat Dent, Aracatuba - Brazil
[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Pediat Dent & Publ Hlth, Aracatuba - Brazil
[3] Univ Campinas UNICAMP, Sch Dent, Dept Restorat Dent, Piracicaba - Brazil
[4] Sao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, Araraquara - Brazil
[5] Nove Julho Univ, Dept Periodontol, Sao Paulo - Brazil
[6] Sao Paulo State Univ UNESP, Inst Chem, Dept Biochem & Chem Technol, Araraquara - Brazil
[7] Univ Campinas UNICAMP, Sch Dent, Dept Oral Diag, Piracicaba - Brazil
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: BIOFOULING; v. 33, n. 10, p. 807-818, 2017.
Citações Web of Science: 5
Resumo

This study evaluated the cytotoxicity and antimicrobial activity of analogs of cationic peptides against microorganisms associated with endodontic infections. L-929 fibroblasts were exposed to LL-37, KR-12-a5 and hBD-3-1C(V) and chlorhexidine (CHX, control), and cell metabolism was evaluated with MTT. The minimal inhibitory concentration (MIC) and the minimal bactericidal/fungicidal concentration (MBC/MFC) of the peptides and CHX were determined against oral pathogens associated with endodontic infections. Enterococcus faecalis and Streptococcus mutans biofilms were cultivated in bovine dentin blocks, exposed to different concentrations of the most efficient antimicrobial peptide and analyzed by confocal laser scanning microscopy. CHX and peptides affected the metabolism of L-929 at concentrations > 31.25 and 500 mu g ml(-1), respectively. Among the peptides, KR-12-a5 inhibited growth of both the microorganisms tested with the lowest MIC/ MBC/MFC values. In addition, KR-12-a5 significantly reduced E. faecalis and S. mutans biofilms inside dentin tubules. In conclusion, KR-12-a5 is a non-cytotoxic agent with potent antimicrobial and antibiofilm activity against oral pathogens associated with endodontic infections. (AU)

Processo FAPESP: 13/24606-5 - Efeito antimicrobiano de análogos de peptídeos catiônicos sobre microrganismos de importância endodôntica e sua influência na expressão gênica de marcadores de mineralização dentinária
Beneficiário:Karina Sampaio Caiaffa
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 12/15346-7 - Peptídeos antimicrobianos: efeito da dimerização e produção de biomembranas de látex/peptídeos
Beneficiário:Eduardo Maffud Cilli
Linha de fomento: Auxílio à Pesquisa - Regular