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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

A Metabolomic Approach to Target Compounds from the Asteraceae Family for Dual COX and LOX Inhibition

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Autor(es):
Chagas-Paula, Daniela A. [1, 2] ; Zhang, Tong [1] ; Da Costa, Fernando B. [2] ; Edrada-Ebel, RuAngelie [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Strathclyde, John Arbuthnott Bldg, 161 Cathedral St, Glasgow G4 0RE, Lanark - Scotland
[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto FCFRP, BR-14040903 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: METABOLITES; v. 5, n. 3, p. 404-430, SEP 2015.
Citações Web of Science: 24
Resumo

The application of metabolomics in phytochemical analysis is an innovative strategy for targeting active compounds from a complex plant extract. Species of the Asteraceae family are well-known to exhibit potent anti-inflammatory (AI) activity. Dual inhibition of the enzymes COX-1 and 5-LOX is essential for the treatment of several inflammatory diseases, but there is not much investigation reported in the literature for natural products. In this study, 57 leaf extracts (EtOH-H2O 7:3, v/v) from different genera and species of the Asteraceae family were tested against COX-1 and 5-LOX while HPLC-ESI-HRMS analysis of the extracts indicated high diversity in their chemical compositions. Using O2PLS-DA (R-2 > 0.92; VIP > 1 and positive Y-correlation values), dual inhibition potential of low-abundance metabolites was determined. The O2PLS-DA results exhibited good validation values (cross-validation = Q(2) > 0.7 and external validation = P-2 > 0.6) with 0% of false positive predictions. The metabolomic approach determined biomarkers for the required biological activity and detected active compounds in the extracts displaying unique mechanisms of action. In addition, the PCA data also gave insights on the chemotaxonomy of the family Asteraceae across its diverse range of genera and tribes. (AU)

Processo FAPESP: 10/10940-2 - Estudos metabolômicos de Asteraceae por CLAE-UV-EM, avaliação do potencial anti-inflamatório "in vitro" e suas correlações através de métodos "in silico".
Beneficiário:Daniela Aparecida Chagas de Paula
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 10/51454-3 - Estudos morfoanatômicos, metabolômicos e moleculares como subsídios à sistemática de espécies de Asteraceae e acesso ao seu potencial farmacológico
Beneficiário:Beatriz Appezzato da Glória
Linha de fomento: Auxílio à Pesquisa - Temático