| Texto completo | |
| Autor(es): |
Maria Isabel de Oliveira
[1]
;
Gislene Mitsue Namiyama
[2]
;
Gabriela Bastos Cabral
[3]
;
João Leandro Ferreira
[4]
;
Noemi Taniwaki
[2]
;
Ana Maria Sardinha Afonso
[1]
;
Isabella Rillo Lima
[1]
;
Luís Fernando Macedo de Brigido
[8]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Adolfo Lutz Inst, Ctr Virol, Nucleo Doencas Resp, Av Dr Arnaldo 355, BR-01246902 Sao Paulo, SP - Brazil
[2] Adolfo Lutz Inst, Nucleo Microscopia Eletron, Sao Paulo, SP - Brazil
[3] Adolfo Lutz Inst, Ctr Virol, Nucleo Doencas Vinculacao Sexual & Sanguinea, Sao Paulo, SP - Brazil
[4] Instituto Adolfo Lutz. Centro de Virologia. Núcleo de Doenças de Vinculação Sexual e Sanguínea - Brasil
[5] Instituto Adolfo Lutz. Centro de Virologia. Núcleo de Doenças de Vinculação Sexual e Sanguínea - Brasil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Revista do Instituto de Medicina Tropical de São Paulo; v. 60, 2018-03-08. |
| Resumo | |
ABSTRACT A great variety of viruses which cause exanthema share other clinical manifestations, making the etiologic identification a very difficult task, relying exclusively on the clinical examination. Rubella virus (RV) infection during the early stages of pregnancy can lead to serious birth defects, known as congenital rubella syndrome (CRS). In the present report, we described the presence of Zika virus (ZIKV) particles in urine samples and also ZIKV isolation in SIRC cells from the urine of a patient in acute phase of suspected rubella disease. The 50-year-old unvaccinated woman living in Sao Paulo, Brazil, was admitted to the emergency room with fever, headache, rash, arthralgia and prostration. Urine samples were collected for virus isolation and RT-qPCR. SIRC and Vero cells were inoculated with urine samples during 7 days. RT-qPCR was performed using measles virus (MV) and RV primers and both were found to be negative. After this result, RT-qPCR was performed for parvovirus B19, herpes virus 6 and ZIKV. The urine sample and the isolate were positive by Real Time PCR for ZIKV and negative for all other viruses tested. The sequences isolated are from the Asiatic lineage. (AU) | |
| Processo FAPESP: | 16/14813-1 - Avaliação do uso de tecnologia de biologia molecular de detecção de viremia plasmática para identificação e incorporação de pacientes na fase aguda da infecção pelo HIV-1 |
| Beneficiário: | Ivana Barros de Campos |
| Modalidade de apoio: | Auxílio à Pesquisa - Pesquisa em Políticas Públicas para o SUS |