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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus

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Author(s):
Maria Isabel de Oliveira [1] ; Gislene Mitsue Namiyama [2] ; Gabriela Bastos Cabral [3] ; João Leandro Ferreira [4] ; Noemi Taniwaki [2] ; Ana Maria Sardinha Afonso [1] ; Isabella Rillo Lima [1] ; Luís Fernando Macedo de Brigido [8]
Total Authors: 8
Affiliation:
[1] Adolfo Lutz Inst, Ctr Virol, Nucleo Doencas Resp, Av Dr Arnaldo 355, BR-01246902 Sao Paulo, SP - Brazil
[2] Adolfo Lutz Inst, Nucleo Microscopia Eletron, Sao Paulo, SP - Brazil
[3] Adolfo Lutz Inst, Ctr Virol, Nucleo Doencas Vinculacao Sexual & Sanguinea, Sao Paulo, SP - Brazil
[4] Instituto Adolfo Lutz. Centro de Virologia. Núcleo de Doenças de Vinculação Sexual e Sanguínea - Brasil
[5] Instituto Adolfo Lutz. Centro de Virologia. Núcleo de Doenças de Vinculação Sexual e Sanguínea - Brasil
Total Affiliations: 5
Document type: Journal article
Source: Revista do Instituto de Medicina Tropical de São Paulo; v. 60, 2018-03-08.
Abstract

ABSTRACT A great variety of viruses which cause exanthema share other clinical manifestations, making the etiologic identification a very difficult task, relying exclusively on the clinical examination. Rubella virus (RV) infection during the early stages of pregnancy can lead to serious birth defects, known as congenital rubella syndrome (CRS). In the present report, we described the presence of Zika virus (ZIKV) particles in urine samples and also ZIKV isolation in SIRC cells from the urine of a patient in acute phase of suspected rubella disease. The 50-year-old unvaccinated woman living in Sao Paulo, Brazil, was admitted to the emergency room with fever, headache, rash, arthralgia and prostration. Urine samples were collected for virus isolation and RT-qPCR. SIRC and Vero cells were inoculated with urine samples during 7 days. RT-qPCR was performed using measles virus (MV) and RV primers and both were found to be negative. After this result, RT-qPCR was performed for parvovirus B19, herpes virus 6 and ZIKV. The urine sample and the isolate were positive by Real Time PCR for ZIKV and negative for all other viruses tested. The sequences isolated are from the Asiatic lineage. (AU)

FAPESP's process: 16/14813-1 - Evaluation of molecular biology technology for plasma viremia detection to the identification and incorporation of patients at acute phase of HIV-1 infection
Grantee:Ivana Barros de Campos
Support Opportunities: Research Grants - Research in Public Policies for the National Health Care System (PP-SUS)