Burkholderia cepacia, cystic fibrosis and outcomes following lung transplantation: experiences from a single center in Brazil

Autor(es)

Danila de Souza Carraro ^[1] ; Rafael Medeiros Carraro ^[2] ; Silvia Vidal Campos ^[2] ; Leandro Ryuchi Iuamoto ^[1] ; Karina Andrighetti de Oliveira Braga ^[3] ; Lea Campos de Oliveira ^[4] ; Ester Cerdeira Sabino ^[5] ; Flavia Rossi ^[6] ; Paulo Manuel Pêgo-Fernandes ^[9]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Div Cirurgia Torac, Inst Coracao InCor, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Div Pneumol, Inst Coracao InCor, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Fac Med FMUSP, Cardiopneumol, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Fac Med FMUSP, Lab Invest ed LIM3, Sao Paulo, SP - Brazil

^[5] Fundacao Pro Sangue Hemoctr Sao Paulo, Secretaria Saude Estado Sao Paulo, Dept Biol Mol, Div Sorol, Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Microbiol, Div Lab Cent, Sao Paulo, SP - Brazil

^[7] Universidade de São Paulo. Faculdade de Medicina FMUSP. Cardiopneumologia - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Clinics; v. 73, 2018-03-12.

Resumo

OBJECTIVES: To evaluate the impact of Burkholderia cepacia complex colonization in cystic fibrosis patients undergoing lung transplantation. METHODS: We prospectively analyzed clinical data and respiratory tract samples (sputum and bronchoalveolar lavage) collected from suppurative lung disease patients between January 2008 and November 2013. We also subtyped different Burkholderia cepacia complex genotypes via DNA sequencing using primers against the recA gene in samples collected between January 2012 and November 2013. RESULTS: From 2008 to 2013, 34 lung transplants were performed on cystic fibrosis patients at our center. Burkholderia cepacia complex was detected in 13 of the 34 (38.2%) patients. Seven of the 13 (53%) strains were subjected to genotype analysis, from which three strains of B. metallica and four strains of B. cenocepacia were identified. The mortality rate was 1/13 (7.6%), and this death was not related to B. cepacia infection. CONCLUSION: The results of our study suggest that colonization by B. cepacia complex and even B. cenocepacia in patients with cystic fibrosis should not be considered an absolute contraindication to lung transplantation in Brazilian centers. (AU)

• Processo FAPESP: 11/51832-0 - Estudo das cepas de burkholderia cepacia complex em pacientes candidatos ao transplante pulmonar:avaliacao dos marcadores de virulencia analise de genomovar.
• Beneficiário: Paulo Manuel Pêgo-Fernandes
• Modalidade de apoio: Auxílio à Pesquisa - Regular