Assessment of the genotoxic/clastogenic potential of coumarin derivative 6,7-dihydroxycoumarin (aesculetin) in multiple mouse organs

Autor(es)

Marques, Eduardo de Souza ^[1] ; Salles, Daiane Bernardoni ^[2] ; Maistro, Edson Luis ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Inst Biociencias, Programa Posgrad Biol Geral & Aplicada, Botucatu, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Fac Filosofia & Ciencias, Dept Fonoaudiol, Av Hygino Muzzi Filho, 737, Caixa Postal 181, BR-17525900 Marilia, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TOXICOLOGY REPORTS; v. 2, p. 268-274, 2015.
• Citações Web of Science: 5

Resumo

6,7-Dihydroxycoumarin (6,7-HC) (aesculetin) is a natural and synthetic coumarin derivative of great interest for use by humans due to their potent antioxidant properties. Considering that there are no reports that assess the in vivo genetic toxicity of 6,7-HC, the aim of the present study was to investigate its genotoxic potential in terms of DNA damage in peripheral blood, liver, bone marrow and testicular cells of Swiss albino mice by the comet assay, and its clastogenic/aneugenic potential in bone marrow cells using the micronucleus test. In addition, the ability of 6,7-HC to modulate the genotoxic effects induced by doxorubicin (DXR) was also preliminarily evaluated. Cytotoxicity was assessed by scoring polychromatic (PCE) and normochromatic (NCE) erythrocytes' ratio. The test compound was administered orally at doses of 25, 50 and 500 mg kg(-1) isolated and also simultaneously to DXR (80 mg kg-1). The results showed that 6,7-HC did not induce significant DNA damage in any of the analyzed cells, and also did not show any significant increase in micronucleated PCE at the three tested doses. The PCE/NCE ratio indicated no cytotoxicity. Moreover, the extent of DNA damage induced by DXR decreased significantly only in peripheral blood and testicular cells, and only at the lowest dose of 6,7-HC. (C) 2015 The Authors. Published by Elsevier Ireland Ltd. This is an open access article under the CC BY-NC-ND license (AU)

• Processo FAPESP: 10/07577-3 - Investigação do potencial genotóxico do artesunato, da artemisinina, da 4-hidroxicumarina esculetina: implantação da metodologia de mutagênese in vitro
• Beneficiário: Edson Luis Maistro
• Modalidade de apoio: Auxílio à Pesquisa - Regular