A Low-Cost Label-Free AFB(1) Impedimetric Immunosensor Based on Functionalized CD-Trodes

Autor(es)

Foguel, Marcos Vinicius ^[1] ; Giordano, Gabriela Furlan ^[1] ; de Sylos, Celia Maria ^[2] ; Carlos, Iracilda Zeppone ^[3] ; Pupim Ferreira, Antonio Aparecido ^[1] ; Benedetti, Assis Vicente ^[1] ; Yamanaka, Hideko ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Inst Chem, Dept Analyt Chem, Rua Francisco Degni 55, BR-14800060 Araraquara, SP - Brazil

^[2] UNESP Univ Estadual Paulista, Dept Food & Nutr, Sch Pharmaceut Sci, Rodovia Araraquara Jau, Km 01 S-N, Campos Ville, BR-14800903 Araraquara, SP - Brazil

^[3] UNESP Univ Estadual Paulista, Dept Clin Anal, Sch Pharmaceut Sci, Rodovia Araraquara Jau, Km 01 S-N, Campos Ville, BR-14800903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CHEMOSENSORS; v. 4, n. 3 SEP 2016.
• Citações Web of Science: 4

Resumo

This work describes the investigation of a label-free immunosensor for the detection of aflatoxin B-1 (AFB(1)). CD-trodes (electrodes obtained from recordable compact disks) were used as low-cost and disposable transducers after modification with a self-assembled monolayer (SAM) of lipoic acid. The anti-aflatoxin B1 antibody was immobilized via EDC/NHS activation, followed by blocking with bovine serum albumin and immunoassays with AFB(1). The optimization of analytical parameters and the detection were carried out using electrochemical impedance measurements. Using chemometric tools, the best conditions for the immunosensor development were defined as: anti-AFB(1) antibody at 1:2000 dilution and surface blocking with 0.5% bovine serum albumin, both incubated for 1 h, and antibody-antigen immunoreaction for 30 min. The impedimetric immunosensor showed a linear range from 5 x 10(-9) to 1 x 10(-7) mol . L-1 (1.56-31.2 ng . mL(-1)), limit of detection and limit of quantification, respectively, 3.6 x 10(-10) and 1.1 x 10(-9) mol . L-1 (0.11 and 0.34 ng.mL(-1)). The proposed immunosensor was applied to analyze peanut samples. (AU)

• Processo FAPESP: 08/07729-8 - Desenvolvimento de imunossensor para aflatoxina B1
• Beneficiário: Marcos Vinicius Foguel
• Modalidade de apoio: Bolsas no Brasil - Mestrado