| Full text | |
| Author(s): |
Foguel, Marcos Vinicius
[1]
;
Giordano, Gabriela Furlan
[1]
;
de Sylos, Celia Maria
[2]
;
Carlos, Iracilda Zeppone
[3]
;
Pupim Ferreira, Antonio Aparecido
[1]
;
Benedetti, Assis Vicente
[1]
;
Yamanaka, Hideko
[1]
Total Authors: 7
|
| Affiliation: | [1] UNESP Univ Estadual Paulista, Inst Chem, Dept Analyt Chem, Rua Francisco Degni 55, BR-14800060 Araraquara, SP - Brazil
[2] UNESP Univ Estadual Paulista, Dept Food & Nutr, Sch Pharmaceut Sci, Rodovia Araraquara Jau, Km 01 S-N, Campos Ville, BR-14800903 Araraquara, SP - Brazil
[3] UNESP Univ Estadual Paulista, Dept Clin Anal, Sch Pharmaceut Sci, Rodovia Araraquara Jau, Km 01 S-N, Campos Ville, BR-14800903 Araraquara, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | CHEMOSENSORS; v. 4, n. 3 SEP 2016. |
| Web of Science Citations: | 4 |
| Abstract | |
This work describes the investigation of a label-free immunosensor for the detection of aflatoxin B-1 (AFB(1)). CD-trodes (electrodes obtained from recordable compact disks) were used as low-cost and disposable transducers after modification with a self-assembled monolayer (SAM) of lipoic acid. The anti-aflatoxin B1 antibody was immobilized via EDC/NHS activation, followed by blocking with bovine serum albumin and immunoassays with AFB(1). The optimization of analytical parameters and the detection were carried out using electrochemical impedance measurements. Using chemometric tools, the best conditions for the immunosensor development were defined as: anti-AFB(1) antibody at 1:2000 dilution and surface blocking with 0.5% bovine serum albumin, both incubated for 1 h, and antibody-antigen immunoreaction for 30 min. The impedimetric immunosensor showed a linear range from 5 x 10(-9) to 1 x 10(-7) mol . L-1 (1.56-31.2 ng . mL(-1)), limit of detection and limit of quantification, respectively, 3.6 x 10(-10) and 1.1 x 10(-9) mol . L-1 (0.11 and 0.34 ng.mL(-1)). The proposed immunosensor was applied to analyze peanut samples. (AU) | |
| FAPESP's process: | 08/07729-8 - Development of immunosensor for aflatoxin B1 |
| Grantee: | Marcos Vinicius Foguel |
| Support Opportunities: | Scholarships in Brazil - Master |