| Texto completo | |
| Autor(es): |
Penteado, Joao C. T.
[1]
;
Borduchi, Rodolpho J.
[1]
;
Maldonado, Mariangela B. C.
[1]
;
Sangalli, Juliano R.
[2]
;
de Bem, Tiago H. C.
[2]
;
Meirelles, Flavio V.
[2]
;
Arnold, Daniel R.
[3]
;
Lopes, Flavia L.
[1]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Sao Paulo State Univ Unesp, Sch Vet Med, BR-16050680 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Anim Sci & Food Engn FZEA, BR-13635000 Pirassununga, SP - Brazil
[3] Vitro Brasil SA, BR-13800970 Sao Paulo - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Animal Reproduction Science; v. 187, p. 174-180, DEC 2017. |
| Citações Web of Science: | 0 |
| Resumo | |
Embryonic and placental development is highly orchestrated by epigenetic processes. Disruptions in normal placental development, commonly observed in pregnancies produced by nuclear transfer, are associated with abnormal gene expression and altered epigenetic regulation of imprinted and vital placental genes. The objective of this study was to evaluate expression and epigenetic regulation of the imprinted gene TSSC4 in cotyledonary and intercotyledonary tissues from day 60 pregnancies produced by embryo transfer (ET), in vitro fertilization (IVF) and nuclear transfer (NT) in cattle. TSSC4 expression was reduced by 30% in cotyledons at 60 days of gestation in the NT group. The proximal promoter region of TSSC4 showed an increase in the permissive histone mark (H3K4me2) and a reduction in the inhibitory histone mark (H3K9me2) in the cotyledons produced by NT, in relation to cotyledons produced by embryo transfer. Interestingly, H3K9me2 was also significantly reduced in cotyledons produced by IVF, compared to the ET controls. DNA methylation, in CpG-rich regions located at the proximal promoter region and the coding region of TSSC4 did not differ. These results suggest that the reduction in TSSC4 expression, observed following NT, can not be explained by the histone changes investigated in the proximal promoter region of the gene, or by changes in methylation in three regions evaluated. Also, a decrease in the levels of H3K9 dimethylation in IVF samples, indicate that in vitro culturing could corroborate with the alterations seen in the NT group. (AU) | |
| Processo FAPESP: | 09/50381-5 - Regulação epigenética do desenvolvimento placentário em bovinos |
| Beneficiário: | Flavia Lombardi Lopes |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |