Systematic identification of novel regulatory interactions controlling biofilm formation in the bacterium Escherichia coli

Autor(es)

Amores, Gerardo Ruiz ^[1] ; de las Heras, Aitor ^{[2, 3]} ; Sanches-Medeiros, Ananda ^[1] ; Elfick, Alistair ^{[2, 3]} ; Silva-Rocha, Rafael ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, FMRP, Ribeirao Preto, SP - Brazil

^[2] Univ Edinburgh, Sch Engn, Inst Bioengn, Edinburgh, Midlothian - Scotland

^[3] Univ Edinburgh, SynthSys Res Ctr, Edinburgh, Midlothian - Scotland

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 7, DEC 1 2017.
• Citações Web of Science: 3

Resumo

Here, we investigated novel interactions of three global regulators of the network that controls biofilm formation in the model bacterium Escherichia coli using computational network analysis, an in vivo reporter assay and physiological validation experiments. We were able to map critical nodes that govern planktonic to biofilm transition and identify 8 new regulatory interactions for CRP, IHF or Fis responsible for the control of the promoters of rpoS, rpoE, flhD, fliA, csgD and yeaJ. Additionally, an in vivo promoter reporter assay and motility analysis revealed a key role for IHF as a repressor of cell motility through the control of FliA sigma factor expression. This investigation of first stage and mature biofilm formation indicates that biofilm structure is strongly affected by IHF and Fis, while CRP seems to provide a fine-tuning mechanism. Taken together, the analysis presented here shows the utility of combining computational and experimental approaches to generate a deeper understanding of the biofilm formation process in bacteria. (AU)

• Processo FAPESP: 15/22386-3 - Construção e caracterização de promotores constitutivos mutantes e de promotores controlados pelos reguladores globais CRP e Fis em Escherichia coli
• Beneficiário: Ananda Sanches Medeiros
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/22921-8 - Abordagens de biologia sintética para decifrar os mecanismos de integração de sinais em promotores bacterianos complexos
• Beneficiário: Rafael Silva Rocha
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores