Crystal structure and insights into the oligomeric state of UDP-glucose pyrophosphorylase from sugarcane

Autor(es)

Cotrim, Camila A. ^{[1, 2, 3]} ; Soares, Jose Sergio M. ^[4] ; Kobe, Bostjan ^{[1, 2]} ; Menossi, Marcelo ^[4]

Afiliação do(s) autor(es)

^[1] Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld - Australia

^[2] Univ Queensland, Australia Infect Dis Res Ctr, Brisbane, Qld - Australia

^[3] Griffith Univ, Griffith Inst Drug Discovery, Brisbane, Qld - Australia

^[4] Univ Estadual Campinas, Inst Biol, Dept Genet Evolucao Microbiol & Imunol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 13, n. 3 MAR 1 2018.
• Citações Web of Science: 3

Resumo

UDP-glucose pyrophosphorylase (UGPase) is found in all organisms and catalyses the formation of UDP-glucose. In sugarcane, UDP-glucose is a branch-point in the carbon channelling into other carbohydrates, such as sucrose and cellulose, which are the major factors for sugarcane productivity. In most plants, UGPase has been described to be enzymatically active in the monomeric form, while in human and yeast, homo-octamers represent the active form of the protein. Here, we present the crystal structure of UGPase from sugarcane (ScUGPase-1) at resolution of 2.0 angstrom. The crystals of ScUGPase-1 reveal the presence of two molecules in the asymmetric unit and the multi-angle light scattering analysis shows that ScUGPase-1 forms a mixture of species ranging from monomers to larger oligomers in solution, suggesting similarities with the orthologs from yeast and human. (AU)

• Processo FAPESP: 13/15576-5 - Abordagens moleculares para estudar a relação entre etileno e maturação em cana-de-açúcar
• Beneficiário: Marcelo Menossi Teixeira
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 08/06767-3 - Caracterização estrutural e funcional de enzimas envolvidas na biossíntese e acúmulo de sacarose em cana-de-açúcar
• Beneficiário: José Sérgio de Macedo Soares
• Modalidade de apoio: Bolsas no Brasil - Doutorado