HPLC-DAD, ESI-MS/MS, and NMR of Lycopene Isolated From P-guajava L. and Its Biotechnological Applications

Autor(es):Mostrar menos -

Amorim, Adriany G. N. ^[1] ; Souza, Jessica M. T. ^[1] ; Santos, Raimunda C. ^[1] ; Gullon, Beatriz ^[2] ; Oliveira, Ana ^[3] ; Santos, Luiz F. A. ^[4] ; Virgino, Adamor L. E. ^[5] ; Mafud, Ana C. ^[6] ; Petrilli, Helena M. ^[5] ; Mascarenhas, Yvonne P. ^[6] ; Delerue-Matos, Cristina ^[7] ; Pintado, Manuela E. ^[3] ; Leite, Jose R. S. A. ^[8]

Afiliação do(s) autor(es)

^[1] Univ Fed Piaui, Ctr Biodivers Res & Biotechnol, Sao Sebastiao Ave 2819, BR-64202020 Parnaiba, Piaui - Brazil

^[2] Univ Santiago de Compostela, Dept Chem Engn, Inst Technol, Santiago De Compostela 15782 - Spain

^[3] Portuguese Catholic Univ, Ctr Biotechnol & Fine Chem, Arquiteto Lobao Vital Apartado St 2511, Porto - Portugal

^[4] Bruker Brasil Ltda, BBP Condominium, Rod D Pedro 1, Km 87-5 Northern Runway, BR-12954260 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Inst Phys, Matao St, Byst R, 187 Block C, Room 102, BR-05508090 Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Sao Carlos Phys Inst, BR-13566590 Sao Paulo - Brazil

^[7] Polytech Inst Porto, REQUIMTE, Super Engn Inst Porto, Porto - Portugal

^[8] Univ Brasilia UnB, Fac Med, Morphol Area, Univ Campus Darcy Ribeiro, BR-70910900 Brasilia, DF - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY; v. 120, n. 3 MAR 2018.
• Citações Web of Science: 3

Resumo

Psidium guajava L. have been reported to be a rich source of antioxidant compounds. Its carotenoids have been highlighted by their high antioxidant capacity, which offers several benefits for human health. In this sense, lycopene isomers need to be identified. In this work, the comprehensive chemical characterization, by HPLC-DAD, MS/MS, and NMR, of lycopene isolated from P. guajava L., antioxidant and antimicrobial activity of lycopene extracts and isolated lycopene are evaluated. The FTIR results reported a structure with Z configuration, confirmed by UV-Vis, with max=448, 473, and 505nm for 5-Z lycopene. Furthermore, MS/MS positive ionization shows one fragment m/z 309 {[}M-227](+), relatively abundant for isolated lycopene. Experimental and Theoretical NMR studies revealed that guava may contain 5-Z lycopene because of the similarity found among the peaks. Lycopene extracts presented higher antioxidant activity than isolated lycopene, from both P. guajava L. and tomato, when measured by ABTS and ORAC (r(2)=0.9995 and r(2)=0.9992, respectively). In addition, lycopene extract shows antibacterial efficacy against E. coli, S. aureus, and L. innocua, presenting MBC values of 20mgmL(-1). These results suggest that lycopene extract have potential applicability for food, cosmetics, and pharmaceutical industry.Practical Applications: Lycopene from P. guajava L. is characterized by HPLC-DAD, MS, NMR, FTIR, and X-Ray, presented antioxidant capacity by ORAC and antibacterial efficacy against food pathogens. Lycopene is isolated from P. guajava L., and lycopene extracts produced are characterized by HPLC-DAD and ESI-MS/MS. Lycopene isolated from P. guajava L. following the process may be 5-Z isomer of lycopene. (AU)

• Processo FAPESP: 14/02282-6 - Estudo da relação estrutura-atividade de alcalóides derivados da Pilocarpus microphyllus (Rutaceae) com ação contra Schistosoma mansoni
• Beneficiário: Ana Carolina Mafud
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado