SPM-1-producing Pseudomonas aeruginosa ST277 clone recovered from microbiota of migratory birds

Autor(es)

Martins, Willames M. B. S. ^[1] ; Narciso, Ana Clara ^[1] ; Cayo, Rodrigo ^[1] ; Santos, Stefanie Vanessa ^[1] ; Fehlberg, Lorena C. C. ^[1] ; Ramos, Patricia Locosque ^[2] ; da Cruz, Joao Batista ^[3] ; Gales, Ana Cristina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Internal Med, Div Infect Dis, Lab Alerta, Sao Paulo - Brazil

^[2] Fundacao Parque Zool Sao Paulo, Dept Pesquisas Aplicadas, Sao Paulo, SP - Brazil

^[3] FPZSP, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 90, n. 3, p. 221-227, MAR 2018.
• Citações Web of Science: 1

Resumo

The production of Sao Paulo metallo-O-lactamase (SPM-1) is the most common carbapenem resistance mechanism detected among multidrug-resistant Pseudomonas aeruginosa clinical isolates in Brazil. Dissemination of SPM-1-producing P. aeruginosa has been restricted to the nosocomial settings, with sporadic reports of environmental isolates due to contamination by hospital sewage. Herein, we described the detection and molecular characterization of SPM-1-producing P. aeruginosa recovered from the microbiota of migratory birds in Brazil. Three hundred gram-negative bacilli were recovered from cloacal and choanal swabs of Dendrocygna viduata during a surveillance study for detection of carbapenem-resistant isolates. All isolates were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing. MICs were determined by agar dilution, except for polymyxin B. Antibiotic resistance genes were detected by polymerase chain reaction (PCR) followed by DNA sequencing. Transcriptional levels of oprD and efflux system encoding genes were also carried out by quantitative real-time PCR. Nine imipenem-resistant P. aeruginosa isolates were recovered with 7 of them carrying bla(SPM-1). Additional resistance genes (rmtD-1, bla(OXA-56), aacA4, and aac(6')-Ib-cr) were also detected in all 9 isolates. The SPM-1-producing isolates showed high MICs for all beta-lactams, fluoroquinolones, and aminoglycosides, being susceptible only to polymyxin B. Interestingly, all isolates showed the same PFGE pattern and belonged to ST277. Overexpression of MexXY-OprM and MexAB-OprM was observed in those isolates that did not harbor bla(SPM-1). Our results suggest that migratory birds might have played a role in the dissemination of SPM-1-producing P. aeruginosa within the Brazilian territory. (C) 2017 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 14/12224-3 - Avaliação dos mecanismos de resistência aos betalactâmicos em isolados clínicos de Burkholderia cenocepacia recuperados de hemocultura
• Beneficiário: Lorena Cristina Corrêa Fehlberg
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/02258-6 - Avaliação do papel da AVE migratória Dendrocygna viduata na disseminação de bacilos gram-negativos resistentes a antimicrobianos
• Beneficiário: Ana Cristina Gales
• Linha de fomento: Auxílio à Pesquisa - Regular