Signatures of protein expression revealed by secretome analyses of cancer associated fibroblasts and melanoma cell lines

Autor(es)

Liberato, Tarcisio ^[1] ; Pessotti, Dayelle S. ^[1] ; Fukushima, Isabella ^[1] ; Kitano, Eduardo S. ^[2] ; Serrano, Solange M. T. ^[2] ; Zelanis, Andre ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Paulo ICT UNIFESP, Dept Sci & Technol, Funct Prote Lab, Sao Jose Dos Campos, SP - Brazil

^[2] Inst Butantan, Immune Response & Cell Signaling CeTICS, Ctr Toxins, Lab Especial Toxinol Aplicada, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROTEOMICS; v. 174, p. 1-8, MAR 1 2018.
• Citações Web of Science: 3

Resumo

The imbalance of cellular homeostasis during oncogenesis together with the high heterogeneity of tumor-associated stromal cells have a marked effect on the repertoire of the proteins secreted by malignant cells (the secretome). Hence, the study of tumoral secretomes provides insights for understanding the cross-talk between cells within the tumor microenvironment as well as the key effectors for the establishment of the pre-metastatic niche in distant tumor sites. In this context, we performed a proteomic analysis of the secretomes derived from four cell lines: a paired set of fibroblasts - Hs 895. T, a cell line obtained from a lung node metastatic site from a patient who had melanoma and Hs 895.Sk, a skin fibroblast cell line (derived from the same patient); two malignant metastatic melanoma cell lines - A375, a malignant melanoma cell line from primary source and SH-4, a cell line derived from pleural effusion of a patient with metastatic melanoma. Clustering of expression profiles together with functional enrichment analysis resulted in patterns that mirrored each cell type. In addition, these patterns might be the result of cell-specific protein expression programs and reveal the emergence of trends in the co-expression of functionally related proteins in cellular melanoma models. (AU)

• Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
• Beneficiário: Hugo Aguirre Armelin
• Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 15/18096-0 - Otimização de técnicas de enriquecimento Nterminal para análise proteômica em larga escala de amostras complexas
• Beneficiário: Isabella Fukushima
• Linha de fomento: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 17/07897-7 - Análise do processamento proteolítico no secretoma de células tumorais de melanoma
• Beneficiário: Isabella Fukushima
• Linha de fomento: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 14/06579-3 - Análise sistêmica do processamento N-terminal e diversidade de proteínas no secretoma de células tumorais
• Beneficiário: André Zelanis Palitot Pereira
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores