Antagonists of Wnt/-catenin signalling in the periodontitis associated with type 2 diabetes and smoking

Autor(es)

Miranda, Tamires S. ^[1] ; Napimoga, Marcelo H. ^[2] ; Feres, Magda ^[1] ; Marins, Leticia M. ^[1] ; da Cruz, Daniele F. ^[1] ; da Silva, Helio Doyle P. ^[1] ; Duarte, Poliana M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Guarulhos, Dent Res Div, Dept Periodontol, Sao Paulo - Brazil

^[2] Sao Leopoldo Mand Inst & Res Ctr, Lab Immunol & Mol Biol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CLINICAL PERIODONTOLOGY; v. 45, n. 3, p. 293-302, MAR 2018.
• Citações Web of Science: 6

Resumo

AimThis study evaluated the levels of sclerostin (SOST) and Dickkopf (DKK)-1 in the chronic periodontitis (CP) associated with type 2 diabetes (DM) and/or smoking. Relationships between SOST, DDK1, RANKL, OPG, IL-1 ss, IL-6 and TNF-, and pathogens were assessed. Material and MethodsThe study population included non-diabetic non-smokers (control), non-smokers with DM (DM group), non-diabetic smokers (S group) and smokers with DM (SDM group), all with CP. Serum and gingival levels of SOST, DKK1, RANKL, OPG, IL-1 ss, IL-6 and TNF- were evaluated by multiplex immunoassay. Gene expressions of these biomarkers and subgingival levels of pathogens were assessed by qPCR. ResultsGingival protein and/or mRNA levels of DKK1 and SOST were higher in subjects with DM and/or smoking than in controls (p<.05). Serum levels of SOST were higher in the DM group than in controls (p<.05). DKK1 positively correlated with SOST in the DM, SDM and control groups (p<.05) at mRNA levels. DKK-1 and SOST correlated with pathogens, especially in both groups with DM. ConclusionsSOST and DKK1 were upregulated in patients with CP presenting DM and/or smoking. DM, alone or with smoking, particularly influenced the correlations of SOST and DKK1 with each other and with the other biomarkers mostly at mRNA levels, as well as with periodontal pathogens. (AU)

• Processo FAPESP: 13/10354-4 - Influência dos fatores de risco para periodontite crônica no perfil imunoinflamatório sistêmico e periodontal
• Beneficiário: Poliana Mendes Duarte
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/23743-9 - Envolvimento dos antagonistas da sinalização Wnt/beta-catenina na periodontite crônica associada a fatores de risco
• Beneficiário: Tamires Szeremeske de Miranda
• Modalidade de apoio: Bolsas no Brasil - Doutorado