The effect of dental bleaching on pulpal tissue response in a diabetic animal model: a study of immunoregulatory cytokines

Autor(es)

Ferreira, L. L. ^[1] ; Gomes-Filho, J. E. ^[1] ; Benetti, F. ^[1] ; Carminatti, M. ^[1] ; Ervolino, E. ^[2] ; Briso, A. L. F. ^[3] ; Cintra, L. T. A. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ Unesp, Sch Dent, Dept Endodont, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Aracatuba, SP - Brazil

^[3] Sao Paulo State Univ Unesp, Sch Dent, Dept Restorat Dent, Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Endodontic Journal; v. 51, n. 3, p. 347-356, MAR 2018.
• Citações Web of Science: 8

Resumo

AimTo evaluate the influence of tooth bleaching on immunoregulatory cytokines production (IL-6, Tumour necrosis factor (TNF)- and IL-17) in the pulp tissue of normoglycaemic and diabetic rats. MethodologyTwenty-eight rats were divided into normoglycaemic and diabetic rats (n=14). Diabetes mellitus (DM) was induced with a single dose of alloxan diluted in citrate buffer via intramuscular injection. After DM confirmation, all rats were sedated and tooth bleaching was performed using 35% hydrogen peroxide on the right maxillary molars for 30min. Left molars were used as controls. Bleaching resulted in four hemimaxillae groups: normoglycaemic (N), N-bleached (NBle), diabetic (D) and D-bleached (DBle). After 2 and 30days, rats were euthanized and hemimaxillae processed for analysis by haematoxylin and eosin and immunohistochemistry. Results within and between animals were submitted to Wilcoxon signed-rank and Mann-Whitney tests (P<0.05). ResultsAt 2days, the NBle group had mild, and the DBle had severe inflammatory infiltration in the pulpal tissue (P<0.05). TNF- and IL-6 cytokines were associated with increased immunolabelling in the bleached groups compared to nonbleached (P<0.05). However, IL-17 had increased immunolabelling in the NBle compared to the N and DBle group (P<0.05). At 30days, reactionary dentine was observed in the coronal pulp of all bleached teeth and no inflammation was present (P>0.05). TNF- cytokines had increased immunolabelling in the DBle group compared to the D group (P<0.05). However, for IL-6 and IL-17, no difference was observed in this period (P>0.05). ConclusionsTooth bleaching increased IL-6 and TNF- in the pulp tissue regardless of diabetes mellitus; however, diabetic rats had higher TNF- levels for longer periods. Tooth bleaching influenced the increase in IL-17 in the early periods in normoglycaemic rats. (AU)

• Processo FAPESP: 13/25429-0 - Presença de caspase, PCNA e CD5 em polpas de ratos submetidos ao tratamento clareador com peróxido de hidrogênio em diferentes concentrações: estudo histológico e imunoistoquímico
• Beneficiário: Francine Benetti
• Modalidade de apoio: Bolsas no Brasil - Mestrado