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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The effect of dental bleaching on pulpal tissue response in a diabetic animal model: a study of immunoregulatory cytokines

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Author(s):
Ferreira, L. L. [1] ; Gomes-Filho, J. E. [1] ; Benetti, F. [1] ; Carminatti, M. [1] ; Ervolino, E. [2] ; Briso, A. L. F. [3] ; Cintra, L. T. A. [1]
Total Authors: 7
Affiliation:
[1] Sao Paulo State Univ Unesp, Sch Dent, Dept Endodont, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil
[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Aracatuba, SP - Brazil
[3] Sao Paulo State Univ Unesp, Sch Dent, Dept Restorat Dent, Aracatuba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: International Endodontic Journal; v. 51, n. 3, p. 347-356, MAR 2018.
Web of Science Citations: 8
Abstract

AimTo evaluate the influence of tooth bleaching on immunoregulatory cytokines production (IL-6, Tumour necrosis factor (TNF)- and IL-17) in the pulp tissue of normoglycaemic and diabetic rats. MethodologyTwenty-eight rats were divided into normoglycaemic and diabetic rats (n=14). Diabetes mellitus (DM) was induced with a single dose of alloxan diluted in citrate buffer via intramuscular injection. After DM confirmation, all rats were sedated and tooth bleaching was performed using 35% hydrogen peroxide on the right maxillary molars for 30min. Left molars were used as controls. Bleaching resulted in four hemimaxillae groups: normoglycaemic (N), N-bleached (NBle), diabetic (D) and D-bleached (DBle). After 2 and 30days, rats were euthanized and hemimaxillae processed for analysis by haematoxylin and eosin and immunohistochemistry. Results within and between animals were submitted to Wilcoxon signed-rank and Mann-Whitney tests (P<0.05). ResultsAt 2days, the NBle group had mild, and the DBle had severe inflammatory infiltration in the pulpal tissue (P<0.05). TNF- and IL-6 cytokines were associated with increased immunolabelling in the bleached groups compared to nonbleached (P<0.05). However, IL-17 had increased immunolabelling in the NBle compared to the N and DBle group (P<0.05). At 30days, reactionary dentine was observed in the coronal pulp of all bleached teeth and no inflammation was present (P>0.05). TNF- cytokines had increased immunolabelling in the DBle group compared to the D group (P<0.05). However, for IL-6 and IL-17, no difference was observed in this period (P>0.05). ConclusionsTooth bleaching increased IL-6 and TNF- in the pulp tissue regardless of diabetes mellitus; however, diabetic rats had higher TNF- levels for longer periods. Tooth bleaching influenced the increase in IL-17 in the early periods in normoglycaemic rats. (AU)

FAPESP's process: 13/25429-0 - Presence of caspase, PCNA and CD5 in the pulp of rats subjected bleaching with different concentrations hydrogen peroxide: study histological and immunohistochemical
Grantee:Francine Benetti
Support Opportunities: Scholarships in Brazil - Master