Pfaffia paniculata (Brazilian ginseng) extract modulates Mapk and mucin pathways in intestinal inflammation

Autor(es)

Costa, C. A. R. A. ^[1] ; Quaglio, A. E. V. ^[1] ; Di Stasi, L. C. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Pharmacol, Lab Phytomed Pharmacol & Biotechnol PhytoPharmaTe, POB 510, BR-18618970 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Ethnopharmacology; v. 213, p. 21-25, MAR 1 2018.
• Citações Web of Science: 2

Resumo

Ethnopharmacological relevance: Pfaffia paniculata is an endemic Brazilian plant traditionally used against fatigue, stress, inflammation and low immune system as well as with proven intestinal anti-inflammatory activity. Aim of the study: To evaluate intestinal anti-inflammatory effects of P. paniculata on the mRNA abundance of Hsp70, Heparanase, Mapk1, Mapk3, Mapk6, Mapk9, Mud., Muc2, Muc3, Muc4, and NF-kappa B, as well as the mucin content in colonic samples. Material and methods: Intestinal inflammation was induced by TNBS and rats were divided into groups that received vehicle or 25, 50, 100, or 200 mg/kg of P. paniculata extract, p.o., started 2 h after inflammation induction and continued daily for 7 days. At the end of the procedure, the animals were killed and their colon samples were obtained for RT-qPCR analysis and mucin histochemical study with PAS/Alcian blue stain. The inflammatory process was confirmed with colon macroscopic analysis and myeloperoxidase (MPO) activity. Results: P. paniculata at 200 mg/kg significantly decreased macroscopic damage score, extension of lesion and colonic MPO activity. Besides, P. paniculata at a dose of 25 mg/kg was also able to significantly decrease Hsp70, while treatment with 50 mg/kg reduced Mapk3 and increased Muc4. At dose of 100 mg/kg P. paniculata increased Mapkl, Muc3, Muc4, and decreased Mapk3. Finally, at the 200 mg/kg P. paniculata reduced Mapk3. The heparanase, Mapk6, Mapk9, Mucl and Muc2 mRNA abundances were not altered after P. paniculata treatments. Conclusion: Intestinal anti-inflammatory activity of P. paniculata was related to modulation of Mapks and mucin gene expression, as well as mucus secretion in intestinal inflammation. (AU)

• Processo FAPESP: 11/50847-4 - Caracterizacao da droga vegetal pfaffia paniculata como adaptogena e antiinflamatoria intestinal.
• Beneficiário: Celso Acácio Rodrigues de Almeida Costa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/15267-8 - Doença inflamatória intestinal (DII): novas abordagens de diagnóstico e modulação da microbiota intestinal em pacientes com retocolite ulcerativa
• Beneficiário: Luiz Claudio Di Stasi
• Modalidade de apoio: Auxílio à Pesquisa - Temático