Morphological Characterization of the Myenteric Plexus of the Ileum and Distal colon of Dogs Affected by Muscular Dystrophy

Autor(es)

Schafer, Barbara Tavares ^[1] ; Silveira, Mariana Povoa ^[1] ; Palombit, Kelly ^{[2, 3]} ; Mendes, Cristina Eusebio ^[2] ; Watanabe, Ii Sei ^[2] ; Miglino, Maria Angelica ^[1] ; Castelucci, Patricia ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Anat, Inst Biomed Sci, R Dr Lineu Prestes 2415, BR-05508900 Sao Paulo - Brazil

^[3] Univ Fed Piaui, Deparment Morphol, Teresina, PI - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology; v. 301, n. 4, p. 673-685, APR 2018.
• Citações Web of Science: 1

Resumo

Duchenne-like muscular dystrophy (canine dystrophinopathy) is a hereditary degenerative disease characterized by muscle changes similar to those described for Duchenne muscular dystrophy (DMD) and by alterations in the smooth muscles of the gastrointestinal tract. Some authors have suggested that these abnormalities may be associated with intestinal motility. This study analyzed the nitrergic and cholinergic neurons and P2X7 receptor expression in the myenteric plexus of the ileum and distal colon of dogs with muscular dystrophy. Immunohistochemical techniques were used to detect nitric oxide synthase (NOS) and acetylcholine transferase (ChAT) expression and to label all HuC/D- and P2X7 receptor-immunoreactive (IR) neurons. Transmission electron microscopy and basic histology were performed for further analysis. The results showed that nitrergic neurons exhibited a Dogiel type I morphology in the ileum and distal colon. The neuronal profile results showed that there were fewer NOS-, ChAT-, and HuC/D-IR neurons in the ileum than in the distal colon in the dystrophic (DT) dogs. Additionally, there were more NOS-, ChAT- and HuC/D-IR neurons per ganglion in the distal colon than in the ileum. The P2X7 receptor-expressing neurons colocalized with nitrergic and cholinergic neurons. Transmission and light microscopy revealed collagen between the muscle fibers, between the circular and longitudinal muscle layers and within the myenteric ganglia of dogs with muscular dystrophy. These findings provide a morphological description of the myenteric neurons in the ileum and distal colon of these DT dogs and may contribute to a better understanding of the gastrointestinal disorders found in patients with DMD. Anat Rec, 301:673-685, 2018. (c) 2017 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 12/00259-1 - Papel do antagonista brilliant blue green (BBG) sobre o receptor P2X7 e o sistema nervoso entérico do íleo de ratos submetidos a isquemia e reperfusão intestinal: estudo morfofuncional
• Beneficiário: Patricia Castelucci
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/05314-5 - Estudo do código químico e da expressão dos receptores P2X2 E P2X7 no sistema nervoso entérico do íleo de ratos submetidos a isquemia/reperfusão intestinal de artérias ileais
• Beneficiário: Patricia Castelucci
• Modalidade de apoio: Auxílio à Pesquisa - Regular