Modeling the Interplay Between Neurons and Astrocytes in Autism Using Human Induced Pluripotent Stem Cells

Autor(es)

Russo, Fabiele Baldino ^{[1, 2]} ; Freitas, Beatriz Camille ^{[3, 4]} ; Pignatari, Graciela Conceicao ^[1] ; Fernandes, Isabella Rodrigues ^{[2, 3, 4]} ; Sebat, Jonathan ^[5] ; Muotri, Alysson Renato ^{[3, 4]} ; Baleeiro Beltrao-Braga, Patricia Cristina ^{[1, 2, 6]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Dept Surg, Sch Vet Med, Sao Paulo, SP - Brazil

^[3] Rady Childrens Hosp San Diego, Dept Pediat, San Diego, CA - USA

^[4] Univ Calif San Diego, Sch Med, Dept Cellular & Mol Med, Stem Cell Program, Sanford Consortium Regenerat Me, La Jolla, CA 92093 - USA

^[5] Univ Calif San Diego, Dept Psychiat Cellular & Mol Med, La Jolla, CA 92093 - USA

^[6] Univ Sao Paulo, Dept Obstet, Sch Arts Sci & Humanities, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOLOGICAL PSYCHIATRY; v. 83, n. 7, p. 569-578, APR 1 2018.
• Citações Web of Science: 19

Resumo

BACKGROUND: Autism spectrum disorder (ASD) is a neurodevelopmental disorder with unclear etiology and imprecise genetic causes. The main goal of this work was to investigate neuronal connectivity and the interplay between neurons and astrocytes from individuals with nonsyndromic ASD using induced pluripotent stem cells. METHODS: Induced pluripotent stem cells were derived from a clinically well-characterized cohort of three individuals with nonsyndromic ASD sharing common behaviors and three control subjects, two clones each. We generated mixed neural cultures analyzing synaptogenesis and neuronal activity using a multielectrode array platform. Furthermore, using an enriched astrocyte population, we investigated their role in neuronal maintenance. RESULTS: ASD-derived neurons had a significant decrease in synaptic gene expression and protein levels, glutamate neurotransmitter release, and, consequently, reduced spontaneous firing rate. Based on co-culture experiments, we observed that ASD-derived astrocytes interfered with proper neuronal development. In contrast, control-derived astrocytes rescued the morphological neuronal phenotype and synaptogenesis defects from ASD neuronal co-cultures. Furthermore, after identifying interleukin-6 secretion from astrocytes in individuals with ASD as a possible culprit for neural defects, we were able to increase synaptogenesis by blocking interleukin-6 levels. CONCLUSIONS: Our findings reveal the contribution of astrocytes to neuronal phenotype and confirm previous studies linking interleukin-6 and autism, suggesting potential novel therapeutic pathways for a subtype of individuals with ASD. This is the first report demonstrating that glial dysfunctions could contribute to nonsyndromic autism pathophysiology using induced pluripotent stem cells modeling disease technology. (AU)

• Processo FAPESP: 11/20683-0 - Geração de células pluripotentes induzidas de pacientes com transtorno autista
• Beneficiário: Fabiele Baldino Russo
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/02978-6 - Modelagem celular de pacientes com Transtorno do Espectro do Autismo através de células-tronco pluripotentes induzidas (iPSC)
• Beneficiário: Fabiele Baldino Russo
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado