Genomic diversity is similar between Atlantic Forest restorations and natural remnants for the native tree Casearia sylvestris Sw.

Autor(es):Mostrar menos -

Gomes Viana, Joao Paulo ^[1] ; Bohrer Monteiro Siqueira, Marcos Vinicius ^[2] ; Araujo, Fabiano Lucas ^[3] ; Grando, Carolina ^[1] ; Sujii, Patricia Sanae ^[1] ; Silvestre, Ellida de Aguiar ^[1] ; Novello, Mariana ^[1] ; Pinheiro, Jose Baldin ^[4] ; Cavallari, Marcelo Mattos ^[5] ; Brancalion, Pedro H. S. ^[6] ; Rodrigues, Ricardo Ribeiro ^[7] ; de Souza, Anete Pereira ^[8] ; Catchen, Julian ^[9] ; Zucchi, Maria I. ^[10]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Grad Program Genet & Mol Biol, Campinas, SP - Brazil

^[2] Univ Sagrado Coracao, Bauru, SP - Brazil

^[3] Agron Inst Campinas, Grad Program Trop & Subtrop Agr, Campinas, SP - Brazil

^[4] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Genet, Piracicaba, SP - Brazil

^[5] EMBRAPA, Embrapa Pecuaria Sudeste, Sao Carlos, SP - Brazil

^[6] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Forest Sci, Piracicaba, SP - Brazil

^[7] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Biol, Piracicaba, SP - Brazil

^[8] Univ Estadual Campinas, Dept Plant Biol, Campinas, SP - Brazil

^[9] Univ Illinois, Dept Anim Biol, Champaign, IL - USA

^[10] Agencia Paulista Tecnol Agronegocios, Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 13, n. 3 MAR 7 2018.
• Citações Web of Science: 2

Resumo

The primary focus of tropical forest restoration has been the recovery of forest structure and tree taxonomic diversity, with limited attention given to genetic conservation. Populations reintroduced through restoration plantings may have low genetic diversity and be genetically structured due to founder effects and genetic drift, which limit the potential of restoration to recover ecologically resilient plant communities. Here, we studied the genetic diversity, genetic structure and differentiation using single nucleotide polymorphisms (SNP) markers between restored and natural populations of the native tree Casearia sylvestris in the Atlantic Forest of Brazil. We sampled leaves from approximately 24 adult individuals in each of the study sites: two restoration plantations (27 and 62 years old) and two forest remnants. We prepared and sequenced a genotyping-by-sequencing library, SNP markers were identified de novo using Stacks pipeline, and genetic parameters and structure analyses were then estimated for populations. The sequencing step was successful for 80 sampled individuals. Neutral genetic diversity was similar among restored and natural populations (A(R) = 1.72 +/- 0.005; H-O = 0.135 +/- 0.005; H-E = 0.167 +/- 0.005; F-IS = 0.16 +/- 0.022), which were not genetically structured by population subdivision. In spite of this absence of genetic structure by population we found genetic structure within populations but even so there is not spatial genetic structure in any population studied. Less than 1% of the neutral alleles were exclusive to a population. In general, contrary to our expectations, restoration plantations were then effective for conserving tree genetic diversity in human-modified tropical landscapes. Furthermore, we demonstrate that genotyping-by-sequencing can be a useful tool in restoration genetics. (AU)

• Processo FAPESP: 11/50296-8 - Biologia da conservação de espécies nativas da Mata Atlântica com potencial fitoterápico: uma abordagem genética sobre restaurações florestais
• Beneficiário: Maria Imaculada Zucchi
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 16/05765-3 - Análise genômica de dados populacionais em Casearia sylvestre de diferentes fitofisionomias brasileiras
• Beneficiário: João Paulo Gomes Viana
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 13/05762-6 - Genômica da conservação em Casearia sylvestris no cerrado e na Mata Atlântica do Sudeste do Brasil
• Beneficiário: João Paulo Gomes Viana
• Modalidade de apoio: Bolsas no Brasil - Doutorado