Genomic diversity is similar between Atlantic Forest restorations and natural remnants for the native tree Casearia sylvestris Sw.

Author(s):Show less -

Gomes Viana, Joao Paulo ^[1] ; Bohrer Monteiro Siqueira, Marcos Vinicius ^[2] ; Araujo, Fabiano Lucas ^[3] ; Grando, Carolina ^[1] ; Sujii, Patricia Sanae ^[1] ; Silvestre, Ellida de Aguiar ^[1] ; Novello, Mariana ^[1] ; Pinheiro, Jose Baldin ^[4] ; Cavallari, Marcelo Mattos ^[5] ; Brancalion, Pedro H. S. ^[6] ; Rodrigues, Ricardo Ribeiro ^[7] ; de Souza, Anete Pereira ^[8] ; Catchen, Julian ^[9] ; Zucchi, Maria I. ^[10]

Affiliation

^[1] Univ Estadual Campinas, Grad Program Genet & Mol Biol, Campinas, SP - Brazil

^[2] Univ Sagrado Coracao, Bauru, SP - Brazil

^[3] Agron Inst Campinas, Grad Program Trop & Subtrop Agr, Campinas, SP - Brazil

^[4] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Genet, Piracicaba, SP - Brazil

^[5] EMBRAPA, Embrapa Pecuaria Sudeste, Sao Carlos, SP - Brazil

^[6] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Forest Sci, Piracicaba, SP - Brazil

^[7] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Biol, Piracicaba, SP - Brazil

^[8] Univ Estadual Campinas, Dept Plant Biol, Campinas, SP - Brazil

^[9] Univ Illinois, Dept Anim Biol, Champaign, IL - USA

^[10] Agencia Paulista Tecnol Agronegocios, Piracicaba, SP - Brazil

• Document type: Journal article
• Source: PLoS One; v. 13, n. 3 MAR 7 2018.
• Web of Science Citations: 2

Abstract

The primary focus of tropical forest restoration has been the recovery of forest structure and tree taxonomic diversity, with limited attention given to genetic conservation. Populations reintroduced through restoration plantings may have low genetic diversity and be genetically structured due to founder effects and genetic drift, which limit the potential of restoration to recover ecologically resilient plant communities. Here, we studied the genetic diversity, genetic structure and differentiation using single nucleotide polymorphisms (SNP) markers between restored and natural populations of the native tree Casearia sylvestris in the Atlantic Forest of Brazil. We sampled leaves from approximately 24 adult individuals in each of the study sites: two restoration plantations (27 and 62 years old) and two forest remnants. We prepared and sequenced a genotyping-by-sequencing library, SNP markers were identified de novo using Stacks pipeline, and genetic parameters and structure analyses were then estimated for populations. The sequencing step was successful for 80 sampled individuals. Neutral genetic diversity was similar among restored and natural populations (A(R) = 1.72 +/- 0.005; H-O = 0.135 +/- 0.005; H-E = 0.167 +/- 0.005; F-IS = 0.16 +/- 0.022), which were not genetically structured by population subdivision. In spite of this absence of genetic structure by population we found genetic structure within populations but even so there is not spatial genetic structure in any population studied. Less than 1% of the neutral alleles were exclusive to a population. In general, contrary to our expectations, restoration plantations were then effective for conserving tree genetic diversity in human-modified tropical landscapes. Furthermore, we demonstrate that genotyping-by-sequencing can be a useful tool in restoration genetics. (AU)

• FAPESP's process: 11/50296-8 - Conservation biology of Atlantic Forest’s medicinal species: a genetical approach to Forest restorations
• Grantee: Maria Imaculada Zucchi
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
• FAPESP's process: 16/05765-3 - Genomic data analysis of Casearia Sylvestris in different Brazilian Biomes
• Grantee: João Paulo Gomes Viana
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 13/05762-6 - Conservation genomic of Casearia sylvestris in Cerrado and Mata Atlântica from Southest region of Brazil
• Grantee: João Paulo Gomes Viana
• Support Opportunities: Scholarships in Brazil - Doctorate