Infra-specific chemical variability and dynamics of phenolic and diterpenic secondary metabolites in Casearia sylvestris Sw (Salicaceae) from different Brazilian ecosystems: metabolomics and proteomics correlation

• Grant number: 11/21440-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2012
• End date: August 31, 2015
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Alberto Jose Cavalheiro
• Grantee: Paula Carolina Pires Bueno
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 12/21921-4 - Proteomics of two varieties of Casearia sylvestris Sw. (Salicaceae), BE.EP.DR

Abstract

The species Casearia sylvestris Sw (Salicaceae) is an important representative of the Casearia Jacq. genus, due to its chemical, pharmacological, economic, environmental and biotechnological aspects. Because of its high adaptive capacity, this species is widespread in Central and South America and, in Brazil, it occurs in practically all biomes. The morpho-anatomical, chemical and genetic data available on C. sylvestris, indicate close relationship between the variety "lingua" and the Cerrado biome, and between the variety "sylvestris" and Atlantic Forest. Also, there are data describing significant genetic differences between these two varieties. In ecotones Cerrado/Atlantic Forest, it can be found these two varieties, including individuals with intermediate characteristics, considering both morpho-anatomical and genetic ones. There is evidence that in C. sylvestris var. "lingua"/Cerrado occur predominantly the phenolic compounds, while in C. sylvestris var. "sylvestris"/Atlantic Forest the clerodane diterpenes predominate. So, taking part in the thematic network CECAA - "Prospecção de moléculas bioativas e estudo de variabilidade infra-específica em plantas e microrganismos endofíticos do Cerrado e Caatinga - Contribuição para o conhecimento e uso sustentável da biodiversidade brasileira." (CNPq 563311/2010-0 and FAPESP 2010/52327-5), the main objective of this project is to evaluate the hypothesis that the chemical composition, considering the secondary metabolites, is related to and / or conditioned by biomes, and mainly associated to their predominant morphotypes and, therefore, under strong genetic control. If this hypothesis is proven, individuals with differential production of clerodane diterpenes (type casearinas) can be used for comparative proteomic studies aiming the identification of enzymes related to the biosynthesis of these important metabolites.

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DANUELLO, AMANDA; DE CASTRO, ROGERIO CARDOSO; PILON, ALAN CESAR; PIRES BUENO, PAULA CAROLINA; PIVATTO, MARCOS; VIEIRA JUNIOR, GERARDO MAGELA; CARVALHO, FLAVIO ALEXANDRE; ODA, FERNANDO BOMBARDA; PEREZ, CONSUELO JAVIERA; LOPES, NORBERTO PEPORINE; et al. Fragmentation study of clerodane diterpenes from Casearia species by tandem mass spectrometry (quadrupole time-of-flight and ion trap). RAPID COMMUNICATIONS IN MASS SPECTROMETRY, v. 34, n. 3, SI, MAY 2020. (11/21440-3, 16/13292-8, 17/19702-6, 13/07600-3, 14/50926-0)

RIBEIRO, SABRINA M.; FRATUCELLI, ERICK D. O.; BUENO, PAULA C. P.; DE CASTRO, V, MARLENE KELLY; FRANCISCO, AMANDA ALCALA; CAVALHEIRO, ALBERTO JOSE; KLEIN, I, MARLISE. Antimicrobial and antibiofilm activities of Casearia sylvestris extracts from distinct Brazilian biomes against Streptococcus mutans and Candida albicans. BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, v. 19, n. 1, NOV 12 2019. (13/07600-3, 14/50926-0, 11/21440-3, 12/21921-4, 17/07408-6)

ANHESINE, NAIRA BUZZO; PIRES BUENO, PAULA CAROLINA; TORRES, ROSELI BUZANELLI; LOPES, NORBERTO PEPORINE; CAVALHEIRO, ALBERTO JOSE. Non-polar and polar chemical profiling of six Casearia species (Salicaceae). Biochemical Systematics and Ecology, v. 87, DEC 2019. (11/21440-3, 13/07600-3, 14/02738-0)

BUENO, PAULA CAROLINA PIRES; SALOME ABARCA, LUIS FRANCISCO; ANHESINE, NAIRA BUZZO; GIFFONI, MAIRA SILVA; PEREIRA, FABIOLA MANHAS VERBI; TORRES, ROSELI BUZANELI; DE SOUSA, RAYRAN WALTER RAMOS; FERREIRA, PAULO MICHEL PINHEIRO; PESSOA, CLAUDIA; CAVALHEIRO, ALBERTO JOSE. Infraspecific Chemical Variability and Biological Activity of Casearia sylvestris from Different Brazilian Biomes. Planta Medica, v. 87, n. 01/02, DEC 2020. (16/20295-3, 12/21921-4, 11/21440-3, 19/01102-8, 14/50945-4, 14/02738-0, 18/18212-8, 13/07600-3)

PIRES BUENO, PAULA CAROLINA; PASSARELI, FERNANDO; ANHESINE, NAIRA BUZZO; TORRES, ROSELI BUZANELLI; CAVALHEIRO, ALBERTO JOSE. Flavonoids from Casearia sylvestris Swartz variety lingua (Salicaceae). Biochemical Systematics and Ecology, v. 68, p. 23-26, OCT 2016. (11/21440-3, 13/07600-3)

RIBEIRO, SABRINA M.; FRATUCELLI, ERICK D. O.; FERNANDES, JULIA M.; BUENO, PAULA C. P.; CAVALHEIRO, ALBERTO JOSE; KLEIN, MARLISE, I. Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, v. N/A, n. 169, p. 23-pg., 2021-03-01. (19/23175-7, 13/07600-3, 17/07408-6, 11/21440-3, 12/21921-4, 14/50926-0)

RIBEIRO, SABRINA M.; FRATUCELLI, ERICK D. O.; FERNANDES, JULIA M.; BUENO, PAULA C. P.; CAVALHEIRO, ALBERTO JOSE; KLEIN, I, MARLISE. Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, n. 169, MAR 2021. (14/50926-0, 13/07600-3, 12/21921-4, 17/07408-6, 19/23175-7, 11/21440-3)

RIBEIRO, SABRINA M.; BUENO, PAULA C. P.; CAVALHEIRO, ALBERTO JOSE; KLEIN, MARLISE I.. Effect of Extracts, Fractions, and Isolated Molecules of Casearia sylvestris to Control Streptococcus mutans Cariogenic Biofilm. ANTIBIOTICS-BASEL, v. 12, n. 2, p. 28-pg., 2023-02-01. (19/23175-7, 11/21440-3)

DANUELLO, AMANDA; DE CASTRO, ROGERIO CARDOSO; PILON, ALAN CESAR; PIRES BUENO, PAULA CAROLINA; PIVATTO, MARCOS; VIEIRA JUNIOR, GERARDO MAGELA; CARVALHO, FLAVIO ALEXANDRE; ODA, FERNANDO BOMBARDA; PEREZ, CONSUELO JAVIERA; LOPES, NORBERTO PEPORINE; et al. Fragmentation study of clerodane diterpenes from Casearia species by tandem mass spectrometry (quadrupole time-of-flight and ion trap). RAPID COMMUNICATIONS IN MASS SPECTROMETRY, v. 34, p. 7-pg., 2020-05-20. (14/50926-0, 16/13292-8, 13/07600-3, 11/21440-3, 17/19702-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BUENO, Paula Carolina Pires. Variabilidade química infraespecífica e dinâmica de metabólitos secundários fenólicos e diterpênicos em Casearia sylvestris Sw. (Salicaceae): correlação metabolômica e proteômica utilizando cromatografia, espectrometria de massas, ressonância magnética nuclear e quimiometria. 2015. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Química. Araraquara Araraquara.