Proteomics of two varieties of Casearia sylvestris Sw. (Salicaceae)

• Grant number: 12/21921-4
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: December 18, 2012
• End date: June 17, 2013
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Alberto Jose Cavalheiro
• Grantee: Paula Carolina Pires Bueno
• Supervisor: Michael Hippler
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Institution abroad: University of Munster, Germany
• Associated to the scholarship: 11/21440-3 - Infra-specific chemical variability and dynamics of phenolic and diterpenic secondary metabolites in Casearia sylvestris Sw (Salicaceae) from different Brazilian ecosystems: metabolomics and proteomics correlation, BP.DR

Abstract

Proteomics is a fundamental technique in the field of the "omics" science, complementing information obtained by the genomic, transcriptomic and metabolomic approaches. The main aim is the study of proteins expressed in a biological system, under certain developmental stage or environmental conditions. Since proteins are the final product of gene expression, indicating the physiological state of an organism, it is expected from this study and, using computational tools for data integration, to understand the chemical and molecular modulation of the phenotype of species in its natural habitat, considering both biotic and abiotic stresses. For the species Casearia sylvestris Sw. (Salicaceae) are recognized two varieties. The variety C. sylvestris sylvestris is mainly characterized by the production of clerodane type diterpenes (casearins) and the variety C. sylvestris lingua produces, unlike the first, mainly phenolic compounds. However, despite the existence of data concerning the chemical composition of these two varieties, as well as some information about the morphological and genetic profile, nothing is known about the profile of the expressed proteins. For this reason, as originally proposed, one of the goals of this project is to apply proteomic studies in order to understand the biosynthetic routes used by the species C. sylvestris using individuals with differentiated production of clerodane type diterpenes (casearins) and phenolic compounds. It is expected to advance in the knowledge of the proteic profile of a brazilian native species, and to integrate this data with already known data about their chemical, genetic and morphological profile, considering the important ecological variability existing. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RIBEIRO, SABRINA M.; FRATUCELLI, ERICK D. O.; BUENO, PAULA C. P.; DE CASTRO, V, MARLENE KELLY; FRANCISCO, AMANDA ALCALA; CAVALHEIRO, ALBERTO JOSE; KLEIN, I, MARLISE. Antimicrobial and antibiofilm activities of Casearia sylvestris extracts from distinct Brazilian biomes against Streptococcus mutans and Candida albicans. BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, v. 19, n. 1, NOV 12 2019. (13/07600-3, 14/50926-0, 11/21440-3, 12/21921-4, 17/07408-6)

BUENO, PAULA CAROLINA PIRES; SALOME ABARCA, LUIS FRANCISCO; ANHESINE, NAIRA BUZZO; GIFFONI, MAIRA SILVA; PEREIRA, FABIOLA MANHAS VERBI; TORRES, ROSELI BUZANELI; DE SOUSA, RAYRAN WALTER RAMOS; FERREIRA, PAULO MICHEL PINHEIRO; PESSOA, CLAUDIA; CAVALHEIRO, ALBERTO JOSE. Infraspecific Chemical Variability and Biological Activity of Casearia sylvestris from Different Brazilian Biomes. Planta Medica, v. 87, n. 01/02, DEC 2020. (16/20295-3, 12/21921-4, 11/21440-3, 19/01102-8, 14/50945-4, 14/02738-0, 18/18212-8, 13/07600-3)

RIBEIRO, SABRINA M.; FRATUCELLI, ERICK D. O.; FERNANDES, JULIA M.; BUENO, PAULA C. P.; CAVALHEIRO, ALBERTO JOSE; KLEIN, MARLISE, I. Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, v. N/A, n. 169, p. 23-pg., 2021-03-01. (19/23175-7, 13/07600-3, 17/07408-6, 11/21440-3, 12/21921-4, 14/50926-0)

RIBEIRO, SABRINA M.; FRATUCELLI, ERICK D. O.; FERNANDES, JULIA M.; BUENO, PAULA C. P.; CAVALHEIRO, ALBERTO JOSE; KLEIN, I, MARLISE. Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, n. 169, MAR 2021. (14/50926-0, 13/07600-3, 12/21921-4, 17/07408-6, 19/23175-7, 11/21440-3)