Mitochondrial bioenergy alterations in avian HD11 macrophages infected with infectious bronchitis virus

Autor(es)

da Silva, Sergio E. L. ^[1] ; Ferreira, Helena L. ^[2] ; Garcia, Andrea F. ^[3] ; Silva, Felipe E. S. ^[4] ; Gameiro, Roberto ^[4] ; Fabri, Carolina U. F. ^[4] ; Vieira, Dielson S. ^[4] ; Cardoso, Tereza C. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Fed Uberlandia, Fac Med Vet FAMEV, Uberlandia, MG - Brazil

^[2] Univ Sao Paulo, FZEA, Dept Vet Med, Pirassununga, SP - Brazil

^[3] Ctr Univ Catolico Salesiano Auxilium, UniSLESIANO, Aracatuba, SP - Brazil

^[4] Univ Sao Paulo State, UNESP, Coll Vet Med, Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF VIROLOGY; v. 163, n. 4, p. 1043-1049, APR 2018.
• Citações Web of Science: 4

Resumo

To establish an association between mitochondrial dysfunction and apoptosis following infectious bronchitis virus (IBV) infection, HD11 avian macrophage cells were infected with the Massachusetts 41 (M41) strain. Our results show that the M41 strain of IBV induced cytopathic effects followed by the release of new viral particles. Elevated numbers of apoptotic cells were observed at 24, 48 and 72 h post-infection (p.i.). Viral infection was associated with mitochondrial membrane depolarization and reactive oxygen species (ROS) production at all of the examined timepoints p.i. In summary, IBV M41 replication in infected HD11 macrophages seems to induce mitochondrial bioenergy failure, acting as a respiratory chain uncoupler, without compromising viral replication. (AU)

• Processo FAPESP: 12/16715-6 - Herpesvirus bovino tipo 5: expressão de genes relacionados à morte celular programada em infecção in vitro
• Beneficiário: Tereza Cristina Cardoso da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular