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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Effects of short-term dietary restriction and glutamine supplementation in vitro on the modulation of inflammatory properties

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Autor(es):
de Oliveira, Dalila C. [1] ; Santos, Ed Wilson [1] ; Nogueira-Pedro, Amanda [1] ; Xavier, Jose Guilherme [2] ; Borelli, Primavera [1] ; Fock, Ricardo Ambrosio [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil
[2] Univ Paulista, Inst Hlth Sci, Sch Vet Med, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: NUTRITION; v. 48, p. 96-104, APR 2018.
Citações Web of Science: 1
Resumo

Objective: Dietary restriction (DR) is a nutritional intervention that exerts profound effects on biochemical and immunologic parameters, modulating some inflammatory properties. Glutamine (GLN) is a conditionally essential amino acid that can modulate inflammatory properties. However, there is a lack of data evaluating the effects of DR and GLN supplementation, especially in relation to inflammatory cytokine production and the expression of transcription factors such as nuclear factor (NF)-kappa B. Methods: We subjected 3-mo-old male Ballb/c mice to DR by reducing their food intake by 30%. DR animals lost weight and showed reduced levels of serum triacylglycerols, glucose, cholesterol, and calcium as well as a reduction in bone density. Additionally, blood, peritoneal, and spleen cellularity were reduced, lowering the number of peritoneal F4/80- and CD86-positive cells and the total number of splenic CD4- and CD8-positive cells. Results: The production of interleukin (IL)-10 and the expression of NF-kappa B in splenic cells were not affected by DR or by GLN supplementation. However, peritoneal macrophages from DR animals showed reduced IL-12 and tumor necrosis factor-alpha production and increased IL-10 production with reduced phosphorylation of NF-kappa B expression. Additionally, GLN was able to modulate cytokine production by peritoneal cells from the control group, although no effects were observed in cells from the DR group. Conclusion: DR induces biochemical and immunologic changes, in particular by reducing IL-12 and tumor necrosis factor-alpha production by macrophages and clearly upregulating IL-10 production, whereas GLN supplementation did not modify these parameters in cells from DR animals. (C) 2017 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 16/16463-8 - Avaliação dos efeitos moduladores e mecanismos do fragmento sintético da leptina LEP 5 na regulação da hematopoiese em camundongos submetidos à desnutrição protéica
Beneficiário:Ricardo Ambrósio Fock
Modalidade de apoio: Auxílio à Pesquisa - Regular