In vitro antibacterial activity of bioactive glass S53P4 on multiresistant pathogens causing osteomyelitis and prosthetic joint infection

Autor(es)

Cunha, Mateus Trinconi ^[1] ; Murca, Maria Aparecida ^[2] ; Nigro, Stanley ^[2] ; Klautau, Giselle Burlamaqui ^[1] ; Salles, Mauro Jose Costa ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Santa Casa de Sao Paulo Sch Med Sci, Dept Internal Med, Div Infect Dis, Sao Paulo - Brazil

^[2] Santa Casa de Sao Paulo Sch Med Sci, Dept Lab Med & Pathol, Sao Paulo - Brazil

^[3] Hosp Irmandade Santa Casa de Misericordia Sao Pau, Rua Dr Cesario Mota Jr 112, BR-01303060 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC INFECTIOUS DISEASES; v. 18, APR 3 2018.
• Citações Web of Science: 1

Resumo

Background: Conventional local treatment for medullary osteomyelitis (OM) includes insertion of antibiotic-loaded polymethylmethacrylate (PMMA) cement. Nevertheless, PMMA may delivery irregular concentration of antibiotic to surrounding tissue. We aimed to compare the in vitro antibacterial activity of Bioactive Glass (BAG) S53P4, which is a compound showing local antibacterial activity, to that of antibiotic-loaded PMMA against multidrug resistant bacteria from OM isolates. Methods: We studied convenience samples of multidrug resistant (MDR) microorganisms obtained from patients presenting OM and prosthetic joint infection (PJI). Mixtures containing tryptic soy broth (TSB) and inert glass beads (2 mm), BAG-S53P4 granules (0.5-0.8 mm and <45 mm) and Gentamicin or Vancomycin-loaded PMMA beads were inoculated with methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative Staphylococcus (MR-CoNS), Pseudomonas aeruginosa or Klebsiella pneumoniae isolates. Glass beads (2.0 mm) were used as a control. Antibacterial activity was evaluated by means of time-kill curve, through seeding the strains on blood agar plates, and subsequently performing colony counts after 24, 48, 72, 96, 120 and 168 h of incubation. Differences between groups were evaluated by means of two-way analysis of variance (ANOVA) and Bonferroni's t test. Results: Inhibition of bacterial growth started soon after 48 h of incubation, reached zero CFU/ml between 120 and 168 h of incubation for both antibiotic-loaded PMMA and BAG S53P4 groups, in comparison with inert glass (p < 0.05). No difference regarding time-kill curves between antibiotic-loaded PMMA and BAG S53P4 was observed. Conclusions: BAG S53P4 presented antibacterial properties as much as antibiotic-loaded PMMA for MDR bacteria producing OM and PJI. (AU)

• Processo FAPESP: 16/20416-5 - Avaliação da atividade antibacteriana in vitro do vidro bioativo (Bioactive glass) sobre patógenos multirresistentes causadores de osteomielite
• Beneficiário: Mateus Trinconi Cunha
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica