A novel Triclosan Methacrylate-based composite reduces the virulence of Streptococcus mutans biofilm

Autor(es)

de Souza Araujo, Isaac Jordao ^[1] ; de Paula, Andreia Bolzan ^[1] ; Bruschi Alonso, Roberta Caroline ^{[2, 3]} ; Taparelli, Jesus Roberto ^[4] ; Innocentini Mei, Lucia Helena ^[4] ; Stipp, Rafael Nobrega ^[5] ; Puppin-Rontani, Regina Maria ^[6]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dent Mat Div, Operat Dent Dept, Piracicaba, SP - Brazil

^[2] Metropolitan Univ Santos UNIMES, Sch Dent, Dept Dent, Santos, SP - Brazil

^[3] Univ Mogi das Cruzes, Sch Dent, Technol Res Ctr, Mogi das Cruzes - Brazil

^[4] Univ Estadual Campinas, Chem Engn Sch, Dept Mat Engn & Bioproc, Campinas, SP - Brazil

^[5] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Oral Diag, Piracicaba, SP - Brazil

^[6] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Pediat Dent, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 13, n. 4 APR 2 2018.
• Citações Web of Science: 2

Resumo

The use of antimicrobial monomers, linked to the polymer chain of resin composites, is an interesting approach to circumvent the effects of bacteria on the dental and material surfaces. In addition, it can likely reduce the incidence of recurrent caries lesions. The aim of this study was to evaluate the effects of a novel Triclosan Methacrylate (TM) monomer, which was developed and incorporated into an experimental resin composite, on Streptococcus mutans (S. mutans) biofilms, focusing on the analyses of vicR, gtfD, gtfC, covR, and gbpB gene expression, cell viability and biofilm characteristics. The contact time between TM-composite and S. mutans down-regulated the gbpB and covR and up-regulated the gtfC gene expression, reduced cell viability and significantly decreased parameters of the structure and characteristics of S. mutans biofilm virulence. The presence of Triclosan Methacrylate monomer causes harmful effects at molecular and cellular levels in S. mutans, implying a reduction in the virulence of those microorganisms. (AU)

• Processo FAPESP: 12/10750-4 - Influência da incorporação de um monômero antimicrobiano a base de triclosan, em um compósito resinoso experimental, na resistência à biodegradação e sobre o biofilme cariogênico
• Beneficiário: Regina Maria Puppin Rontani
• Modalidade de apoio: Auxílio à Pesquisa - Regular