Development of nanoparticles from natural lipids for topical delivery of thymol: Investigation of its anti-inflammatory properties

Autor(es)

Pivetta, Thais P. ^[1] ; Simoes, Sandra ^[2] ; Araujo, Margarete M. ^[1] ; Carvalho, Tania ^[3] ; Arruda, Caroline ^[4] ; Marcato, Priscyla D. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Nanobiolab, Ribeirao Preto - Brazil

^[2] Univ Lisbon, Fac Farm, ULisboa Res Inst Med, Nanostruct Syst Overcoming Biol Barriers Grp iMed, Lisbon - Portugal

^[3] Univ Lisbon, Fac Med, Inst Med Mol, Histol & Comparat Pathol Lab, Lisbon - Portugal

^[4] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 164, p. 281-290, APR 1 2018.
• Citações Web of Science: 23

Resumo

Wound healing involves the integration of biological and molecular events and, in case of chronic wounds, the use of drugs can be associated to side effects. Therefore, there is a search for alternatives therapeutics that encompass minimal toxicity. The use of natural compounds is an attractive approach for treating inflammatory disorders, wounds and burns. In this context, thymol has antimicrobial, antioxidant and antiseptic properties and is a promising compound in wound healing and inflammation management. However, essential oils and their constituents such as thymol present high volatility and can also easily decompose, thereby the encapsulation of these compounds into nanoparticles may be an efficient approach to modulate the release of the active ingredient, to increase the physical stability and to eventually reduce the toxicity. The aims of this work were to encapsulate thymol in nanostructured lipid carriers (NLCs) composed of natural lipids and assess its in vivo anti-inflammatory and antipsoriatic activity. The carrier containing thymol was produced by sonication method and showed 107.7 (+/- 3.8) nm of size, zeta potential of -11.6 (+/- 2.9) mV and entrapment efficiency of 89.1 (+/- 4.2)%. Thymol-NLCs were incorporated into a gel and the final formulation presented rheological characteristics and pH suitable for topic application. In addition, the gel containing thymol-NLCs was tested in vivo on two different mouse models of skin inflammation, showing anti-inflammatory activity. Finally, this formulation was tested in an imiquimod-induced psoriasis mouse model and showed improved healing, compared to negative control. Therefore, thymol-NLCs is an interesting formulation for future treatment of inflammatory skin diseases. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/24413-2 - Desenvolvimento de carreador lipídico nanoestruturado de manteiga de Illipê e óleo de Calêndula como sistema de encapsulamento de quercetina: Avaliação físico-química e biológica
• Beneficiário: Thais Priscilla Pivetta
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/07596-4 - Encapsulação de timol em carreadores lipídicos nanoestruturados compostos por lipídios naturais
• Beneficiário: Thais Priscilla Pivetta
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Iniciação Científica