Genomic characterisation of Leptospira inadai serogroup Lyme isolated from captured rat in Brazil and comparative analysis with human reference strain

Autor(es)

Moreno, Luisa Z. ^[1] ; Miraglia, Fabiana ^[2] ; Loureiro, Ana P. ^[2] ; Kremer, Frederico S. ^[3] ; Eslabao, Marcus R. ^[3] ; Dellagostin, Odir A. ^[3] ; Lilenbaum, Walter ^[2] ; Vasconcellos, Silvio A. ^[1] ; Heinemann, Marcos B. ^[1] ; Moreno, Andrea M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Vet & Zootecn, Lab Epidemiol Mol & Resistencia Antimicrobianos, Sao Paulo, SP - Brazil

^[2] Univ Fed Fluminense, Dept Microbiol & Parasitol, Lab Bacteriol Vet, Niteroi, RJ - Brazil

^[3] Univ Fed Pelotas, Ctr Desenvolvimento Tecnol, Pelotas, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 113, n. 5 2018.
• Citações Web of Science: 0

Resumo

Leptospira inadai is classified as a species of the Leptospira intermediate group that has been poorly studied due to its apparent insignificance to human and animal health. Nevertheless, over the last two decades the species has been described in human cases in India and in carrier animals in Ecuador. Here, we present the first identification and genomic characterisation of L. inadai serogroup Lyme isolated from captured rodent in Brazil. Even though the M34/99 strain was not pathogenic for hamsters, it was able to establish renal colonisation. The M34/99 strain presented high similarity with L. inadai serogroup Lyme human reference indicating that animal strain could also infect humans, although it does not represent high risk of severe disease. An extrachromosomal sequence was also identified in M34/99 strain and presented high identity with previously described L. inadai phage LinZ\_10, suggesting that phage-like extrachromosomal sequence may be another feature of this understudied species. (AU)

• Processo FAPESP: 13/17136-2 - Caracterização de estirpes de Leptospira isoladas no Brasil através de tipificação por eletroforese em campo pulsado (PFGE) e análise comparativa de genomas.
• Beneficiário: Luisa Zanolli Moreno
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/18290-0 - Caracterização de estirpes de Leptospira isoladas no Brasil através da tipagem por sequenciamento de multilocus (MLST)
• Beneficiário: Andrea Micke Moreno
• Modalidade de apoio: Auxílio à Pesquisa - Regular