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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Susceptibility of Enterococcus faecalis and Propionibacterium acnes to antimicrobial photodynamic therapy

Texto completo
Autor(es):
de Annunzio, Sarah Raquel [1] ; de Freitas, Laura Marise [1] ; Blanco, Ana Ligia [1] ; da Costa, Mardoqueu Martins [2] ; Carmona-Vargas, Christian C. [3] ; de Oliveira, Kleber Thiago [3] ; Fontana, Carla Raquel [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista Unesp, Fac Ciencias Farmaceut, Rodovia Araraquara Jau, Km 1, Campus Ville, BR-14800903 Araraquara, SP - Brazil
[2] Univ Brasil UniBrasil, Dept Engn Biomed, Rua Carolina Fonseca 235, , BR-08230030 Sao Paulo, SP - Brazil
[3] Univ Fed Scio Carlos UFSCar, Dept Quim, Lab Quim Bioorgan, Rodovia Washington Luis, Km 235 SP-310, BR-13565905 Sao Carlos, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 178, p. 545-550, JAN 2018.
Citações Web of Science: 10
Resumo

Bacterial resistance to available antibiotics nowadays is a global threat leading researchers around the world to study new treatment modalities for infections. Antimicrobial photodynamic therapy (aPDT) has been considered an effective and promising therapeutic alternative in this scenario. Briefly, this therapy is based on the activation of a non-toxic photosensitizing agent, known as photosensitizer (PS), by light at a specific wavelength generating cytotoxic singlet oxygen and free radicals. Virtually all studies related to aPDT involve a huge screening to identify ideal PS concentration and light dose combinations, a laborious and time-consuming process that is hardly disclosed in the literature. Herein, we describe an antimicrobial Photodynamic Therapy (aPDT) study against Enterococcus faecalis and Propionibacterium acnes employing methylene blue, chlorin-e6 or curcumin as PS. Similarities and discrepancies between the two bacterial species were pointed out in an attempt to speed up and facilitate futures studies against those clinical relevant strains. Susceptibility tests were performed by the broth microdilution method. Our results demonstrate that aPDT mediated by the three above-mentioned PS was effective in eliminating both gram-positive bacteria, although P. acnes showed remarkably higher susceptibility to aPDT when compared to E. faecalis. PS uptake assays revealed that P. acnes is 80 times more efficient than E. faecalis in internalizing all three PS molecules. Our results evidence that the cell wall structure is not a limiting feature when predicting bacterial susceptibility to aPDT treatment. (AU)

Processo FAPESP: 16/05345-4 - Otimização da terapia fotodinâmica para doenças infecciosas utilizando o peptídeo aureína 1.2
Beneficiário:Carla Raquel Fontana Mendonça
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 15/21110-4 - Explorando Reações Fotocatalisadas em Condições de Fluxo Contínuo pelo uso de Sistemas Porfirinóides: (C-FlowPhotoChem)
Beneficiário:Kleber Thiago de Oliveira
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/24581-5 - Avaliação do papel da terapia fotodinâmica combinada a peptídeos antimicrobianos frente à resistência bacteriana
Beneficiário:Laura Marise de Freitas
Linha de fomento: Bolsas no Brasil - Doutorado