Moxidectin residues in lamb tissues: Development and validation of analytical method by UHPLC-MS/MS

Autor(es)

Cruz, Michelle Del Bianchi A. ^[1] ; Fernandes, Maria A. M. ^[2] ; Braga, Patricia A. de C. ^[1] ; Monteiro, Alda L. G. ^[2] ; Daniel, Daniela ^[3] ; Reyes, Felix G. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Sch Food Engn, Dept Food Sci, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil

^[2] Univ Fed Parana, Dept Anim Sci, Sheep & Goat Prod & Res Ctr LAPOC, Rua Funcionarios 1540, BR-80035050 Curitiba, Parana - Brazil

^[3] Agilent Technol Brasil, Alameda Araguaia 1142, BR-06455000 Barueri, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 1072, p. 390-396, JAN 1 2018.
• Citações Web of Science: 2

Resumo

The development and validation of a throughput method for the quantitation of moxidectin residues in lamb target tissues (muscle, kidney, liver and fat) was conducted using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). To achieve higher recovery of the analyte from the matrices, a modified QuEChERS method was used for sample preparation. The chromatographic separation was achieved using a Zorbax Eclipse Plus C18 RRHD column with a mobile phase comprising 5 mM ammonium formate solution + 0.1% formic acid (A) and acetonitrile + 0.1% formic acid (B) in a linear gradient program. Method validation was performed based on the Commission Decision 2002/657/EC and VICH GL49. To quantify the analyte, matrix-matched analytical curves were constructed with spiked blank tissues, with a limit of quantitation of 5 ng g(-1) and limit of detection of 1.5 ng g(-1) for all matrices. The linearity, decision limit, detection capability accuracy, and inter- and infra-day repeatability of the method are reported. The method was successfully applied to incurred lamb tissue samples (muscle, liver, kidney and fat) in a concentration range from 5 to 200 mu g kg(-1), which demonstrated its suitability for monitoring moxidectin residues in lamb tissues in health surveillance programs, as well as for pharmacokinetics and residue depletion studies. (AU)

• Processo FAPESP: 13/50452-5 - Veterinary drugs: development and validation of bioanalytical methods using LC-MS/MS and MALDI-TOF-MS and its applications in sanitary surveillance and the development of fish farming in Brazil
• Beneficiário: Felix Guillermo Reyes Reyes
• Modalidade de apoio: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE